复合酶在叶下珠有效成分提取中的应用  

Complex Enzyme-Assisted Extraction of Effective Components from Phyllanthus urinaria L.

作　　者：蓝峻峰[1] 廖政达[1] 谢济运[1] 玉澜[1] 

机构地区：[1]柳州师范高等专科学校化学与生命科学系,广西柳州545004

出　　处：《安徽农业科学》2011年第25期15254-15255,15257,共3页Journal of Anhui Agricultural Sciences

摘　　要：[目的]探索复合酶辅助提取叶下珠有效成分的工艺条件,为叶下珠的高效利用提供依据。[方法]以水为提取溶剂,以总黄酮提取得率为考察指标,选择料液比、提取温度、提取时间、纤维素酶浓度和果胶酶浓度等5因素4水平,用正交试验优选其最佳工艺条件。[结果]复合酶法提取叶下珠有效成分的最佳提取工艺为:提取时间2 h,料液比1∶25(g∶ml),提取温度40℃,纤维素酶浓度1.4 g/L,果胶酶2.0 g/L。在此条件下,黄酮的提取率为1.589%。试验所考察的5个因素中,对提取率影响的主次顺序为:提取温度>果胶酶浓度>料液比>提取时间>纤维酶浓度。[结论]复合酶法提取省时、高效、低碳耗且提取率高,是一种对环境友好的方法。[Objective]To optimize the complex enzyme-assisted extraction process of effective components from Phyllanthus urinaria L.,so as to provide references for its high-efficient utilization.[Methods]The flavonoids in phyllanthus urinaria L.was extracted by using water as the extraction agent.The extraction conditions were optimized by the orthogonal test with 4 levels and 5 factors,namely,material-liquid ratio,extraction temperature,extraction time,the concentration of cellulose and pectase.[Result]The optimum extraction technology was：the extraction time was 2 h,the material-liquid ratio was 1∶ 25,extraction temperature was 40 ℃,the concentration of cellulose was 1.4 g/L and pectase concentration was 2.0 g/L.Under the optimized conditions,the extraction ratio of flavonoids was 1.589%.The influence of the inspected five factors on the extraction yield decreased in the order of extraction temperaturepectase concentrationmaterial-liquid ratioextraction timecellulose concentration.[Conclusion]Complex enzyme-assisted extraction method was rapid,efficient and environmental-friendly with low carbon consumption and high extraction yield.

关 键 词：叶下珠(Phyllanthus urinaria L.) 复合酶 黄酮 提取工艺 

分 类 号：TQ281[化学工程—有机化工]