虾Y-器官7、8-脱氢酶转化胆固醇为7-脱氢胆固醇研究  

Study on the Transformation of Cholesterol to 7-dehydrocholesterol by 7,8-dehydrogenase from Shrimps' Y-organ

作　　者：黄时海[1] 何鑫平[1] 于配配[1] 曹喜秀[1] 曹普美[1] 吴孔阳[1] 李湘萍[2] 

机构地区：[1]广西大学生命科学与技术学院,广西南宁530005 [2]广西大学动物繁殖研究所,广西南宁530005

出　　处：《安徽农业科学》2011年第25期15397-15399,共3页Journal of Anhui Agricultural Sciences

基　　金：广西区教委人才项目(桂教人200962);国家大学生创新基金(091059318);广西大学实验教改项目(092301)

摘　　要：[目的]以虾Y-器官微粒体7、8-脱氢酶催化胆固醇,制备7-脱氢胆固醇。[方法]通过单因素试验,研究影响转化反应的各种因素。[结果]将摘除眼柄的刀额新对虾(Metapenaeus ensis)Y-器官,匀浆、离心制备的微粒体酶液,于22℃与胆固醇避光反应6 h,可以积累相对较多的7-脱氢胆固醇。[结论]该研究为利用虾Y-器官7、8-脱氢酶转化胆固醇为7-脱氢胆固醇奠定基础。[Objective] The research aimed to catalyze cholesterol to prepare 7-dehydrocholesterol by using 7,8-dehydrogenase from shrimps＇ Y-organ microsome.[Method] Using single factor test,various influencing factors of the transformation test were studied.[Result] Y organ of Metapenaeus ensis that eyestalk was removed was used to prepare microsomal enzyme solution after homogenation and centrifugation.It was reacted with cholesterin at 22 ℃ under shading conditions,which could accumulate relatively more 7-dehydrocholesterol.[Conclusion] The research laid the foundation for transforming cholesterol to 7-dehydrocholesterol using 7,8-dehydrogenase from shrimps＇ Y-organ.

关 键 词：虾Y-器官 7、8-脱氢酶 胆固醇 7-脱氢胆固醇 生物转化 

分 类 号：S188[农业科学—农业基础科学]