树舌产糖培养基的选择及树舌粗多糖的纯化  

Medium Selection for the Production of Sugar and Purification of Ganoderma applanatum Crude Polysaccharide

作　　者：郑岚[1,2] 马耀宏[2] 杨俊慧[2] 张秀泉[3] 

机构地区：[1]山东轻工业学院,山东济南250353 [2]山东省科学院生物研究所,山东省生物传感器重点实验室,山东济南250014 [3]济南大学,山东济南250022

出　　处：《食品与发酵工业》2011年第8期112-116,共5页Food and Fermentation Industries

基　　金：山东省自然基金资助项目(Y2007D54);国家糖工程技术研究中心开放课题(NGRC2009F05)

摘　　要：分别用不同的培养基培养树舌,实验结果表明,合成培养基产糖量最高,树舌胞外多糖的产率为61.75mg/100 mL,胞内多糖的产率为0.51 mg/100 mL。树舌多糖纯化的最优纯化方案为:用1.5 g/50 mL活性炭进行脱色,按V(样品)∶V(三氯甲烷)∶V(正丁醇)=25∶5∶1的比例除蛋白,用1.5倍体积乙醇沉淀多糖。按优化的方案纯化树舌粗多糖,纯化后粗多糖中多糖的含量由21.6%上升为33.6%,多糖损耗率为52.7%。Ganoderma applanatum is cultured in different mediums. The results show that synthetic medium has the highest sugar yield. The production of Ganoderma applanatum extracellular polysaccharide is 61.75 mg/100 mL and the intracellular polysaccharide is 0.51 mg/100 mL. The amount of activated carbon for decolorization is 1.5 g/50 mL in purifying ganoderma applanatum crude polysaccharide. The ratio of deproteinization processing is sample： chloroform： butanol =25： 5：1 ; Ethanol of 1.5 volume was used to precipitate the polysaccharide. Ganoderma applanatum crude polysaccharide purification was optimized. After purification, the polysaccharide content in crude polysaccharide is increased from 21.6% to 33.6% , while the polysaccharide is lost 52.7%.

关 键 词：树舌 培养基 多糖 纯化 

分 类 号：TQ929.2[轻工技术与工程—发酵工程]