眉斑并脊天牛纤维素酶性质的研究  

Research on Glenea cantor Cellulase Characteristics

作　　者：杨登峰[1] 关妮[1] 米慧芝[1] 杜奇石[1] 张穗生[1] 黄日波[1] 

机构地区：[1]广西科学院非粮生物质酶解国家重点实验室,国家非粮生物质能源工程技术研究中心,广西生物炼制重点实验室,广西南宁530007

出　　处：《广西科学》2011年第3期261-263,268,共4页Guangxi Sciences

基　　金：广西科学基金项目(桂科青0832016)资助

摘　　要：为了了解眉斑并脊天牛(Glenea cantor)纤维素酶的性质,选取以木棉树(Bombax ceiba)为食的眉斑并脊天牛为研究对象,按照IUPAC的标准测定方法,测定和定位眉斑并脊天牛肠道中的内切-β-1,4葡聚糖酶、纤维二糖酶以及滤纸酶活力,考察内切-β-1,4葡聚糖酶的最适pH值和最适温度。结果表明:在眉斑并脊天牛肠道中同时存在以上3种纤维素酶,3种纤维素酶都具有酶活力,并且主要集中在天牛肠道的中肠。内切-β-1,4葡聚糖酶具有广泛的pH值和温度适应范围,最适pH值为9.2,最适温度为50℃。According to the standard method from IUPAC,the activities of endo-β-1,4-glucanase,cellobiase and filter paper enzyme from Glenea cantor were analyzed and their locations were determined.Optimal pH value and optimal temperature of endo-β-1,4-glucanase were measured.The results showed that there were activities of all above three cellulases in Glenea cantor.They were mainly located in the midgut.The endo-β-1,4-glucanase had activity in a broad pH value and temperature range.Optimal pH value and optimal temperature of the endo-β-1,4-glucanase were 9.2 and 50℃,respectively.

关 键 词：眉斑并脊天牛 纤维素 纤维素酶 

分 类 号：Q55[生物学—生物化学]