白灵菇菌丝体多糖两种测定方法的比较  

Comparison of Two Determination Methods for Polysaccharide Content in Pleurotus eryngii var.nebrodensis

作　　者：王守现[1] 刘宇[1] 赵爽[1] 许峰[1] 耿小丽[1] 王兰青[1] 孟莉莉[1] 

机构地区：[1]北京市农林科学院植保环保研究所,北京100097

出　　处：《食品研究与开发》2011年第9期162-164,共3页Food Research and Development

基　　金：北京市优秀人才项目(20071D020500054);北京市科技新星项目(2009A29)

摘　　要：为选出一种比较准确、方便的测定食用菌菌丝体多糖方法,以白灵菇菌丝体提取的粗多糖为原料,对苯酚-硫酸法、蒽酮-比色法2种方法进行比较。苯酚-硫酸法和蒽酮-硫酸法的稳定性实验RSD分别为0.71%和1.40%,精密度实验RSD分别为2.10%和9.53%,回收率平均值分别125.41%和154.88%;苯酚-硫酸法具有稳定性好、精密度高,而且回收率实验也较为理想,是测定食用菌菌丝体多糖含量较为理想的方法。In order to select an accurate,convenient method of edible fungi mycelia polysaccharide determination,the methods of phenol-sulfuric acid method and Anthrone-sulfuric acid method were compared.The results showed the RSD value of the two methods were 0.71 % and 1.40 % in stability experiment,2.10 % and 9.53 % in accuracy experiment,the average recovery was 125.42 % and 154.88 %,respectively.So phenol-sulfuric acid method was more stable and accurate than Anthrone-sulfuric acid method.The recovery was quite ideal.So the better method to determine edible fungi mycelia polysaccharide content was phenol-sulfuric acid method.

关 键 词：白灵菇 多糖含量 苯酚-硫酸法 蒽酮-硫酸法 

分 类 号：TS219[轻工技术与工程—粮食、油脂及植物蛋白工程]