甘蔗渣活性氧脱木素及其对纤维素酶解的影响  

Effects of Reactive Oxygen on Bagasse Delignification and the Cellulase Hydrolysis

作　　者：谢土均[1] 林鹿[1] 庞春生[1] 杨秋林[1] 施建斌[1] 

机构地区：[1]华南理工大学制浆造纸工程国家重点实验室,广东广州510640

出　　处：《现代食品科技》2011年第9期1069-1073,共5页Modern Food Science and Technology

基　　金：国家自然科学基金(50776035;U0733001);国家科技支撑计划项目(2007BAD34B01);973计划项目(2010CB732201)

摘　　要：本文以温度、氧压、H2O2浓度和镁基氧化物的用量作为单因素实验条件,采用活性氧对甘蔗渣进行脱木素,然后进行纤维素酶解实验。探讨活性氧脱木素的效果及其对酶解效果的影响。结果表明,甘蔗渣经过活性氧脱木素后,木素脱除率高达96.23%,酶水解得率达到52.36%。甘蔗渣经过活性氧预处理后,大量木素脱除,有利于纤维素酶水解。This study established a novel process by using reactive oxygen（H2O2 and O2） delignification of bagasse to explore the effect of lignin content of bagasse.In a typical experiment,the treatment conditions were as follow： temperature of 145 ℃～165 ℃,oxygen pressure of 0 MPa～1.2 MPa,the amount of Mg-based Oxides of 0～10.0 g and concentration of H2O2 of 0～16%.Then the bagasse was used as raw materials for cellulase hydrolysis to investigate the sugar yield of enzymatic hydrolysis.The result showed that the reactive oxygen delignification could effectively enhance the sugar yield of enzymatic hydrolysis of bagasse and delignification rate.The rate of lignin removal was up to 96.23% and the sugar yield of the enzymatic hydrolysis was 52.36%.After delignification with reactive oxygen,a great deal of lignin in bagasse were removed,which was in favor of the enzymatic hydrolysis.

关 键 词：甘蔗渣 预处理 活性氧 酶水解 

分 类 号：TS249.2[轻工技术与工程—制糖工程]