一株海洋细菌对原油降解的分析  

BIODEGRADATION OF CRUDE OIL BY A MARINE BACTERIAL STRAIN

作　　者：王中华[1,2] 李佳霖[1] 李太武[2] 苏秀榕[2] 秦松[1] 

机构地区：[1]中国科学院烟台海岸带研究所,山东烟台264003 [2]宁波大学生命科学与生物工程学院,浙江宁波315211

出　　处：《海洋湖沼通报》2011年第3期89-96,共8页Transactions of Oceanology and Limnology

基　　金：浙江省重大科技攻关项目(2006C13089);中国科学院创新团队国际合作伙伴计划"海岸带典型环境过程和资源效应"资助

摘　　要：对实验室所获得的一株海洋细菌LU1进行原油的降解特性分析和降解条件的优化,结果表明,温度、pH、盐度、油质量浓度、接种量等均对石油的降解率有影响,在25℃,初始pH8.0,含油量为0.5%的低盐条件下,其对原油的降解效果最佳,降解率达61%。将其与实验室分离得到的一株降解率为67%的原油降解微生物DYL-1混合后,其对原油的降解率可达78%,混合菌的降解效果要优于单菌。气相色谱分析结果表明,此菌株能够降解原油组分中的烃类物质,对原油具有较为显著的降解。16S序列分析结果表明此株菌属于假交替单胞菌属(Pseudoalteromonassp.)细菌。The experiments were carried out to analyze degradation characteristics and optimize degradation conditions of crude oil by a marine bacterial strain LU1.The results showed that the oil degradation efficiency was affected by temperature,pH,salinity,oil concentration,and bacterial inoculum size.Thus,the optimum degradation conditions were obtained as follows： the incubation temperature 25℃,pH8,the oil concentration 0.5%（W/W）.Crude oil degradation rate could be up to 61% under the optimum conditions.Mixed with another bacterium DYL-1,the degradation rate was further increased to 78%.GC results showed that the composition of degraded oil was considerably changed especially of the hydrocarbon components,suggested that LU1 had the high degradation capability.Phylogenetic analyses based on the 16S rRNA sequence revealed that strain LU1 belonged to Pseudoalteromonas sp.

关 键 词：原油 生物降解 16S RRNA 降解率 气相色谱 

分 类 号：X172[环境科学与工程—环境科学]