高产胞外葡萄糖-1-磷酸菌株的筛选及鉴定  

Screening and identification of high yield extracellular glucose-1-phosphate strain

作　　者：许向华[1] 史军花[1] 赵国罡[2] 郑鸿雁[1] 

机构地区：[1]吉林农业大学食品科学与工程学院,吉林长春130118 [2]吉林大学农业与生物工程学院,吉林长春130022

出　　处：《中国酿造》2011年第4期102-105,共4页China Brewing

基　　金：吉林农业大学青年启动基金项目(2009371)

摘　　要：采用平板初筛、摇瓶复筛结合TLC和HPLC-MS检测的方法,从有机物丰富的土壤中筛选到一株以麦芽糖为底物直接发酵高产胞外葡萄糖-1-磷酸的菌株;经形态学、生理生化特性及16S rDNA基因序列分析,鉴定该菌株为枯草芽孢杆菌。A high-yielding extracellular glucose-1-phosphate strain with maltose as substrate was isolated from organic-rich soil by plate screening,shaking flask rescreening and combined with thin-layer chromatography and high performance liquid chromatography-mass spectrum detection.Through morphological,physiological and biochemical properties and 16S rDNA gene sequence analysis,it was identified as Bacillus subtilis.

关 键 词：葡萄糖-1-磷酸 筛选 鉴定 

分 类 号：Q93-331[生物学—微生物学]