慢性脑缺血大鼠OXR表达的实验分析  

Study on the OXR expression of chronic cerebral ischemia in rats

作　　者：吕斌[1] 娄季宇[2] 张萍[3] 李文杰[4] 

机构地区：[1]河南省食品药品检验所,郑州450003 [2]郑州大学第二附属医院,郑州450014 [3]郑州市第一人民医院,郑州450002 [4]郑州大学公共卫生学院,郑州450001

出　　处：《中国实用神经疾病杂志》2011年第19期22-25,共4页Chinese Journal of Practical Nervous Diseases

摘　　要：目的探讨慢性脑缺血时食欲素受体(OXR)的表达。方法通过结扎双侧颈总动脉(2VO)建立脑缺血模型,免疫组化测定OXR表达。结果慢性脑缺血是OX1R及OX2R出现了一致的表达变化,即缺血15d时二者表达均明显增加,1个月时下降,2个月时再次升高。缺血1个月时大部分神经细胞萎缩,而2个月时部分细胞恢复正常。结论提示缺血的早期OXR可能有诱导细胞凋亡的作用,而缺血的后期OXR可能有神经保护作用。Objective To study the OXR expression of chronic cerebral ischemia in rat. Methods Cerebral ischemia model was established by 2VO, and the OXR expression was measured by immunohistochemical technique. Results OX1R and OX2R had the similar expression of chronic cerebral ischemia, the OXR expression increased obviously on the 15th day, and decreased on one month after ischemia and increased significantly on two months after ischemia. From the histology, most neurons of one-month model got atrophy, however, the neurons of two month model partially recovered normal. Conclusion OXR maybe induce the apoptosis at the early stage of chronic cerebral ischemia, and may have nerve protection at the late stage.

关 键 词：慢性脑缺血 大鼠 OREXIN OXR表达 

分 类 号：R-332[医药卫生]