反相高效液相色谱法测定茶饮料中儿茶素类化合物和咖啡因  

RP-HPLC Determination of Catechinic Compounds and Caffeine in Tea Drinks

作　　者：李琛[1] 陈悦鸣[1] 李迎丽[1] 

机构地区：[1]长春市疾病预防控制中心,长春130033

出　　处：《理化检验（化学分册）》2011年第9期1080-1082,1085,共4页Physical Testing and Chemical Analysis(Part B:Chemical Analysis)

摘　　要：应用反相高效液相色谱法测定茶饮料中咖啡因（CAF）和5种儿茶素类化合物，包括儿茶素（C）、表儿茶素（EC）、表没食子儿茶素没食子酸酯（EGCG）、表儿茶素没食子酸酯（ECG）及表没食子儿茶素（EGC）。在TGC18色谱柱（4．6mm×250mm，5μm）上进行分离，用甲醇乙腈一乙酸～水（230-H60＋2-1-708）混合溶液为流动相进行淋洗，流量为1．0mL·min，在波长210nm处进行紫外检测。用6种组分的标准溶液制作了各自的标准曲线，所得线性回归方程的相关系数在0．9998～0．9999之间。方法用于茶饮料的分析，6种组分的加标回收率在86．6％～118．1％之间，相对标准偏差（n=6）在0．74％～3．4％o之间。RP-HPLC was applied to the determination of caffeine （CAF） and 5 catechinic compounds, namely： eatechin （C）, epicatechin （EC）, epigallocatechin gallate （ECK2G）, epicatechin gallate （ECG） and epigallocate＇chin （EGC）, in tea drinks. The 6 components were separated on the TC-C18 column （4. 6 mmX 250 mm, 5 gm） by elution with a mixture of methanol-acetonitrile-acetie acid-water （230 ＋ 60 ＋ 2 ＋ 708） as mobile phase. Flow-rate of the mobile phase was set at 1.0 mL · min 1, and UV-detection at 210 nm was adopted in the determination. Standard curves of the 6 components were prepared by using the respective standard solutions, and linear regression equations with correlation coefficients from 0. 9998 to 0. 9999 were obtained. The proposed method was applied to the analysis of tea drinks, giving values of recovery obtained by standard addition method in the range of 86.6%-118. 1% and RSD＇s （n=6） in the range of 0.74%-3.4%.

关 键 词：反相高效液相色谱法 茶饮料 儿茶素类化合物 咖啡因 

分 类 号：O652.63[理学—分析化学]