高粱黑粉菌的分离鉴定  

Isolation and Identification of Sporisorium reilianum

作　　者：路秀丽[1] 姚艳飞[2] 王莘[2] 

机构地区：[1]吉林农业大学食品科学与工程学院,吉林长春130118 [2]吉林农业大学生命科学院,吉林长春130118

出　　处：《食品科学》2011年第17期243-245,共3页Food Science

基　　金：长春市科技局科技支撑计划项目(2008214)

摘　　要：目的:以"吉粮105A"高粱乌米为材料,进行高粱黑粉菌的分离鉴定。方法:利用单胞分离的方法从样品中分离获得纯菌株,对分离菌株进行形态学观察及ITS(internal transcribed spacer,ITS)序列测定。结果:分离得到的菌株在天然固体培养基中形成的菌落表面呈革质,边缘呈锯齿状,棕黑色,ITS扩增片段大小为721bp;利用DNAStar软件对序列进行比较分析,鉴定出所分离菌株为丝孢堆菌。结论:成功从高粱乌米中分离出一株高粱丝孢堆黑粉菌。Objective： To isolate and identify Sporisorium reilianum from sorghum cultivar Jiliang 105A.Methods： Single spores were isolated from samples.The morphology of isolated spores was examined and the internal transcribed spacer（ITS） was sequenced.Results： The strain grown on the natural solid culture medium revealed leather face as well as jagged and brownish-black edge.The amplified fragment length of ITS was 721 bp.Based on sequence alignment carried out using DNAStar software,the isolated strain was identified as Sporisorium reilianum.Conclusion： Sporisorium reilianum is successfully isolated and identified from Jiliang 105A.

关 键 词：黑粉菌 ITS序列 分离 鉴定 

分 类 号：TS201.3[轻工技术与工程—食品科学]