饲粮蛋白质水平对肥育猪肌肉嫩度及背最长肌钙调磷酸酶-活化T细胞核因子信号途径的影响  

Dietary Protein Level Affects the Tenderness and CaN-NFAT Signaling Pathway of Longissimus Dorsi in Finishing Pigs

作　　者：张勇[1] 陶亮[1] 崔岩[1] 朱宇旌[1] 邓科[1] 孙璀[1] 邵彩梅[2] 

机构地区：[1]沈阳农业大学畜牧兽医学院,沈阳110866 [2]辽宁禾丰牧业股份有限公司,沈阳110164

出　　处：《动物营养学报》2011年第7期1153-1160,共8页CHINESE JOURNAL OF ANIMAL NUTRITION

基　　金：国家自然科学基金"钙蛋白酶调控猪体蛋白降解的信号途径研究"(30972112)

摘　　要：本试验旨在研究饲粮理想蛋白质水平对背最长肌嫩度及钙调磷酸酶-活化T细胞核因子(CaN-NFAT)信号途径相关蛋白和钙蛋白酶抑制蛋白(CAST)mRNA表达量的影响。选择初始重约50kg的杜洛克×长白×大白三元杂交猪90头,随机分配到3个处理,每个处理3个重复,每个重复10头,公母各占1/2。3个处理分别采用12%、16%、20%理想蛋白质水平的饲粮,饲粮能量水平相同。正试期58d,结束后屠宰取样,测定肌肉剪切力并利用实时定量PCR法测定猪背最长肌CAST、CaN、钙调蛋白(CaM)和NFAT mRNA表达量。结果表明:1)饲粮高蛋白质水平显著提高背最长肌剪切力(P<0.05)、极显著提高CAST和CaN mRNA表达量(P<0.01)。2)背最长肌剪切力与CAST mRNA表达量正相关(相关系数为0.713,P<0.01);CaN、CaM与CAST mRNA表达量正相关(相关系数分别为0.594、0.596,P<0.05);NFAT、CaM与CaN mRNA表达量正相关(相关系数分别为0.536、0.546,P<0.05);CaM与NFAT mRNA表达量正相关(相关系数为0.623,P<0.05)。结果提示,饲粮高蛋白质水平显著降低背最长肌嫩度、提高CAST和CaN mRNA表达量;背最长肌嫩度与CAST mRNA表达量正相关,与CaN-NFAT信号途径无相关性。The study was conducted to investigate the effects of dietary ideal protein level on tenderness and mRNA expression level of CaN-NFAT signaling pathway related to0020proteins and CAST of longissimus dorsi in finishing pigs.Ninety crossbred pigs（Duroc × Landrace × Large white,about 50 kg BW） were randomly allocated into 3 treatments with 3 replicates and per treatment 10 heads per replicate.Pigs in the three treatments were fed diets with the same energy level and ideal protein levels at 12%,16% and 20%,respectively.All pigs were slaughtered after 58 days experiment and muscle samples were collected for the analysis of shear force,as well as mRNA expression levels of CAST,CaN,CaM and NFAT of longissimus dorsi in finishing pigs were determined by real-time PCR.The results showed as follows： 1） High dietary protein level significantly increased the shear force（P0.05） and mRNA expression levels of CaN and CAST of longissimus dorsi（P0.01）.2） The shear force was positively correlated with the mRNA expression levels of CAST（coefficient correlation=0.713,P0.01）;the mRNA expression levels of NFAT（coefficient correlation=0.536,P0.05） and CaM（coefficient correlation=0.546,P0.05） were positively correlated with that of CaN;the mRNA expression levels of CaM and NFAT were positively correlated（coefficient correlation=0.623,P0.05） to each other.The results indicate that high dietary protein level can significantly decrease the tenderness,but increase mRNA expression levels of CAST and CaN of longissimus dorsi;the tenderness of longissimus dorsi is positively correlated with CAST mRNA expression level,but is not correlated with the CaN-NFAT signaling pathway.

关 键 词：蛋白质水平 钙蛋白酶抑制蛋白 钙调磷酸酶 活化 T细胞核因子 钙调蛋白 肌肉嫩度 

分 类 号：S828[农业科学—畜牧学]