褐藻糖胶抑制人肝癌细胞HepG2增殖的机制  

Mechanism of inhibiting the proliferation of the human hepatoma HepG2 cells by fucoidan

作　　者：李小婷[1] 殷辰俞[1] 孟徐莲[1] 段颉[1] 孔秋月[1] 冯晴[1] 

机构地区：[1]南京医科大学公共卫生学院营养与食品卫生系,江苏南京210029

出　　处：《南京医科大学学报（自然科学版）》2011年第9期1261-1265,共5页Journal of Nanjing Medical University(Natural Sciences)

基　　金：国家自然科学基金面上项目(30972479);江苏省自然科学基金(BK2009421)

摘　　要：目的:探讨褐藻糖胶对人肝癌细胞株HepG2体外增殖的相关机制。方法:采用MTT法测定不同浓度褐藻糖胶对人肝癌细胞株HepG2增殖的抑制作用,计算细胞生长抑制率;将0、10、100、500μg/ml的褐藻糖胶作用于HepG2细胞,48 h后光镜观察细胞形态改变,用Hoechst染色法和琼脂糖凝胶电泳检测细胞凋亡;Western blot检测cyclinD1和拓扑异构酶IIα(topoi-somerase IIα,TopoIIα)表达的改变。结果:褐藻糖胶具有抑制人肝癌细胞HepG2增殖的作用,呈剂量依赖性。不同浓度褐藻糖胶作用HepG2细胞后,500μg/ml组较其他浓度组光镜下和Hoechst 33258染色均呈现较明显细胞形态改变,100μg/ml和500μg/ml组均检测出DNA梯形条带。褐藻糖胶也能降低细胞增殖生物标志蛋白cyclinD1和TopoIIα的蛋白表达。结论:褐藻糖胶抑制人肝癌细胞增殖,诱导细胞凋亡,其机制可能与褐藻糖胶抑制细胞增殖的生物标志蛋白cyclinD1和TopoIIα表达有关。Objective： To explore the effect and the related mechanisms of fucoidan on hepatoma cell proliferation.Methods： Proliferation inhibition rate of hepatoma HepG2 cells was measured by MTT.A variety of dosage of fucoidan（0 μg/ml,10 μg/ml,100 μg/ml and 500 μg/ml） was treated to HepG2 cells.The morphology changes of the cells were observed under microscopy.Apoptosis was detected by Hoechst 33258 staining and DNA Ladder analysis.CyclinD1 and topoisomerase IIα（TopoIIα） were as the proliferation biomarker.Their protein expression levels were examined by Western blot.Results： Fucoidan inhibited HepG2 cells proliferation in a dose-dependent manner.There was a remarkable morphological change when cells were treated with 500 μg/ml of fucoidan.Apoptosis was occurred when the cells were incubated with 100 μg/ml and 500 μg/ml of fucoidan.In addition,fucoidan also suppressed the expression of cyclinD1 and TopoIIα in HepG2 cells.Conclusion： Fucoidan can inhibit the proliferation of HepG2 cells and induce apoptosis.Meanwhile,fucoidan also has the ability to suppress the protein expression of cyclinD1 and topoIIα,which may be involved in the mechanisms of inhibiting hepatoma cells proliferation.

关 键 词：褐藻糖胶 人肝癌细胞HEPG2 凋亡 CYCLIND1 TOPOIIΑ 

分 类 号：R735.7[医药卫生—肿瘤]