片球菌素PediocinPA-1抑菌活性检测  

Antibacterial activity detection of Pediocin PA-1

作　　者：马淑霞[1] 李丽秋[1] 丁思[2] 任继秋[1] 吴庆田[1] 张昆[1] 杨春佳[1] 崔刚[1] 胡新俊[1] 代月[1] 王春敏[1] 

机构地区：[1]佳木斯大学基础医学院微生物教研室,黑龙江佳木斯154007 [2]大庆油田总医院集团,黑龙江大庆163319

出　　处：《中国微生态学杂志》2011年第9期800-801,共2页Chinese Journal of Microecology

基　　金：黑龙江省自然科学基金资助项目(D2007-68)

摘　　要：目的检测通过基因工程获得的片球菌素Pediocin PA-1抑菌活性。方法采用琼脂扩散法检测片球菌素Pediocin PA-1对单核细胞增生李斯特杆菌、金黄色葡萄球菌、铜绿假单胞菌、沙门菌和大肠埃希菌O157的抑菌活性。结果片球菌素Pediocin PA-1对单核细胞增生李斯特杆菌、金黄色葡萄球菌、沙门菌、铜绿假单胞菌和大肠埃希菌O157等均有抑制作用。其中对单核细胞增生李斯特杆菌、沙门菌、大肠埃希菌和金黄色葡萄球菌的抑制作用效果明显,对铜绿假单胞菌有微弱的抑制作用。结论通过基因工程获得的片球菌素Pediocin PA-1具有抑菌活性。Objective To detect the antibacterial activity of Pediocin PA-1 acquired by genetic engineering.Method The antibacterial activity of Pediocin PA-1 against Listeria monocytogenes,Staphylococcus aureus,Pseudomonas aeruginosa,Salmonella and E.coli O157 were detected by agar diffusion method.Result The Pediocin PA-1 had inhibitory effect on Listeria monocytogenes,Staphylococcus aureus,Pseudomonas aeruginosa,Salmonella and E.coli O157,which were obvious on Listeria monocytogenes,Staphylococcus aureus,Salmonella,and E.coli O157,and weak on Pseudomonas aeruginosa.Conclusion The Pediocin PA-1 acquired by genetic engineering has got antibacterial activity.

关 键 词：片球菌素PA-1 抑菌活性 检测 

分 类 号：R378.1[医药卫生—病原生物学]