微波辅助提取山茱萸多糖的工艺优化  

Optimization of the Microwave-assisted Extraction Process of Polysaccharides from FRUCTUS CORNI

作　　者：李豪[1] 车振明[2] 谷燕[1] 刘燕[1] 吴永军[1] 黄维[1] 陈荣华[1] 

机构地区：[1]马鞍山师范高等专科学校理工系,安徽马鞍山243041 [2]西华大学生物工程学院,四川成都610039

出　　处：《安徽农业科学》2011年第26期15998-16000,16003,共4页Journal of Anhui Agricultural Sciences

基　　金：高等学校省级优秀青年人才基金项目(2010SQRL222)

摘　　要：[目的]优化微波辅助提取山茱萸（FRUCTUS CORNI）多糖的提取工艺。[方法]在全面单因素试验的基础上,先采用Plackett-Burman设计对7个因素进行试验,筛选出影响最大的3个因子,并确定其余因素的最优水平,再利用响应面法设计对3个主要因子进行试验和优化,建立了二次多项式数学模型。[结果]微波功率、液料比和乙醇体积分数是主要因子,最佳工艺条件是：山茱萸粉末直径在300~450μm之间,料液比1∶19.7（g∶m l）,微波功率624 W,辐照时间55 s,水浴温度90℃,浸提时间2 h,乙醇体积分数为68.4%,在此条件下,多糖得率达20.94%。[结论]该方法误差小、数据可靠,可用于山茱萸多糖的提取。[Objective] To optimize the microwave-assisted extraction technology of polysaccharides from FRUCTUS CORNI.[Method] Firstly,the most 3 influential factors were selected by Plackett-Burman design test with 7 factors on the basis of single-factor test,and the optimum levels of other factors were determined.Secondly,a quadratic polynomial model was established from experimental data that was obtained from the optimization of three main factors by response surface design.[Result] The microwave power,solid-liquid ratio and ethanol volume fraction were the main factors affecting the extraction of polysaccharides from FRUCTUS CORNI.The optimum extraction conditions were as follows： FRUCTUS CORNI powder between 300-450 μm in diameter,solid-liquid ratio of 1∶19.7（g∶ml）,microwave power of 624 W,irradiation time of 55 s,extraction temperature of 90 ℃,extraction time of 2 h,ethanol volume fraction of 68.4%.Under the above conditions,the yield of polysaccharides from FRUCTUS CORNI reached 20.94%.[Conclusion] The method was less in error,accurate in data,and available to extract polysaccharides from FRUCTUS CORNI.

关 键 词：山茱萸(FRUCTUS CORNI) 多糖 微波辅助提取 优化 

分 类 号：S567.239[农业科学—中草药栽培]