吸附-涂层法固定化磷脂酶Lecitase~ Ultra的研究  

Immobilization of Lecitase~ Ultra by adsorption-coating method and its properties

作　　者：付敏[1] 赵谋明[1] 刘宁[1] 汪勇[2] 

机构地区：[1]华南理工大学轻工与食品学院,广东广州510641 [2]暨南大学食品科学与工程系,广东广州510632

出　　处：《食品工业科技》2011年第10期277-280,共4页Science and Technology of Food Industry

基　　金：国家863计划项目(2010AA101505);国家自然科学基金(31000793)

摘　　要：比较了7种大孔吸附树脂对磷脂酶Lecitase誖Ultra的固定化效果,对固定化条件进行了研究。结果表明,极性树脂DA201为该酶的最适固定化载体,在室温下,以pH7.0,0.01mol/L的Tris-HCl缓冲液为媒介,加酶量30mg/g树脂,采用正硅酸乙酯对经真空干燥的固定化酶进行涂层处理,通过电镜扫描发现其涂层效果较好。在最适条件下得到的固定化酶活力为1250～1300U/g,连续操作5次后酶活保存率为55%。与游离酶比较,固定化磷脂酶Lecitase誖Ultra的热稳定性、pH稳定性均有一定程度的提高。Seven kinds of macroporous resin were employed to immobilize phospholipase Lecitase Ultra,and the immobilization conditions were studied. The results indicated that DA201 was the best carrier. The optimum operation conditions were： under room temperature,pH 7.0 Tris-HCl （0.01mol/L）,enzyme dosage 30mg/g resin. Tetraethyl orthosilicate was then used as the coating reagent for immobilized enzyme by the vacuum drying,which showed an effective coating effect through scanning electron microscope. Under optimized conditions,the immobilized Lecitase Ultra with enzyme activity of 1250-1300U/g could be obtained,and it could remain 55% enzyme activity after continuous operation for 5 times. Compared with free Lecitase Ultra, there was a certain extent improvement for the thermal stability and pH stability of immobilized Lecitase Ultra.

关 键 词：磷脂酶Lecitase ULTRA 大孔吸附树脂 涂层 固定化 稳定性 

分 类 号：TS201.25[轻工技术与工程—食品科学]