大麦虫水溶蛋白的分离纯化及抗氧化性研究  

Isolation,Purification and Antioxidant Activity Evaluation of Water Soluble Protein from Giant Mealworm Beetle(Zophobas morio) Larvae

作　　者：张建新[1] 郭倩[1] 何桂梅[1] 刘娜[1] 

机构地区：[1]西北农林科技大学食品科学与工程学院,陕西杨凌712100

出　　处：《食品科学》2011年第18期30-33,共4页Food Science

摘　　要：目的:研究大麦虫水溶蛋白各组分的抗氧化性。方法:以大麦虫幼虫脱脂粉为原料,采用磷酸盐缓冲液提取大麦虫水溶性蛋白,经过硫酸铵分级沉淀、Sephadex G-100凝胶过滤层析和DEAE-Sepharose FF离子交换层析对大麦虫水溶性蛋白逐步分离纯化,并对各组分的抗氧化性进行研究。结果:大麦虫水溶蛋白,经DEAE-Sepharose FF离子交换层析进一步纯化后得到的蛋白组分对羟自由基和超氧阴离子自由基清除率分别达到99.42%和55.11%。结论:大麦虫水溶蛋白组分P2M2是一类纯度高、抗氧化活性好的蛋白。Objective： To isolate and purify water soluble protein from giant mealworm beetle（Zophobas morio） larvae and evaluate its antioxidant activity.Methods： The water soluble protein in giant mealworm beetle larvae was extracted with pH 7.4 phosphate buffer,purified by ammonium sulfate precipitation,and fractionated by Sephadex G-100 column chromatography to obtain fractions P1,P2 and P3.Fraction P2 was further fractionated by DEAE-Sepharose FF column chromatography into subfractions P2M1,P2M2 and P2M3.The antioxidant activities of each fraction and each subfraction were investigated by DPPH and hydroxyl free radical scavenging assays.Results： Subfraction P2M2 presented the highest DPPH and hydroxyl free radical scavenging rates,which were 99.42% and 55.11%,respectively.

关 键 词：大麦虫 蛋白 分离纯化 抗氧化 

分 类 号：TQ936.2[化学工程]