超临界CO_2萃取猕猴桃根三萜类化合物工艺优化  

Optimization of Supercritical Carbon Dioxide Extraction of Triterpenoids from Radix Actinidiae Chinensis

作　　者：李加兴[1] 孙金玉 陈双平 张朝晖[3] 黄诚[1] 王小勇 

机构地区：[1]吉首大学食品科学研究所,湖南吉首416000 [2]湖南省猕猴桃产业化工程技术研究中心,湖南吉首416000 [3]吉首大学化学化工学院,湖南吉首410006 [4]湘西老爹生物有限公司,湖南吉首410006

出　　处：《食品科学》2011年第18期72-76,共5页Food Science

基　　金："十二五"国家科技支撑计划项目(2011BAD10B01)

摘　　要：以湘西"米良1号"猕猴桃根为原料,采用超临界CO2萃取法,对其三萜类化合物的提取工艺进行优化。通过单因素试验探讨猕猴桃根生长期、粉碎粒度、萃取所用夹带剂种类及用量对三萜类化合物得率的影响,并通过正交试验研究猕猴桃根三萜类化合物的最佳萃取工艺条件。结果表明,采用生长期为3年的猕猴桃根为原料,控制粉碎粒度60目,按照1:3(g/mL)的用量加入70%乙醇溶液作为夹带剂,其三萜类化合物的最佳萃取条件为萃取温度40℃、萃取压力22MPa、萃取时间3h、CO2流速300kg/h,此条件下得率可达1.994%。Radix Actinidiae Chinensis from cultivar Miliang No.1,,grown in west Hunan province was used as the raw material to extract triterpenoids by means of supercritical CO2.The effects of material growth period,material particle size entrainer type,entrainer amount and four extraction parameters on extraction rate of triterpenoids were investigated by one-factor-at-a-time method,and four extraction parameters were optimized by orthogonal array design method.The optimal extraction conditions were determined as follows： material growth period 3 years,particle size 60 mesh,70%（V/V） ethanol as entrainer,material-ethanol ratio 1：3（g/mL）,extraction temperature 40 ℃,extraction pressure 22 MPa,extraction time 3 h,and CO2 flow rate 300 kg/h.Under the optimal conditions,the extraction rate of triterpenoids was 1.994%.

关 键 词：猕猴桃根 三萜类化合物 超临界CO2 夹带剂 萃取工艺 

分 类 号：TS201.1[轻工技术与工程—食品科学]