气相色谱法快速检测食品中的苯甲酸、山梨酸、脱氢乙酸  

Determination of benzoic,sorbic,dehydroacetic acid in foods by gas chromatography

作　　者：徐陆妹[1] 

机构地区：[1]杭州市萧山区疾病预防控制中心,杭州311200

出　　处：《中国卫生检验杂志》2011年第9期2199-2201,共3页Chinese Journal of Health Laboratory Technology

摘　　要：目的:建立一种能快速、简便检测食品中山梨酸、脱氢乙酸、苯甲酸的毛细管柱气相色谱法。方法:用氢氧化钠-硫酸锌体系沉淀样品中的蛋白类杂质,石油醚+乙醚(3+1)去除脂类物质,加盐酸酸化后,乙酸乙酯提取,毛细管气相色谱法检测。结果:浓度在50μg～1000μg时,各组分线性良好r,均>0.9990,1.0 g样品量进行萃取检测时,山梨酸、脱氢乙酸、苯甲酸的样品检出限分别为0.033 g/kg,0.050 g/kg,0.010 g/kg。方法的回收率在90%～102%之间,RSD在0.96%～3.3%之间。结论:本方法快速、简便、结果稳定,适合大批量样品的检测。Objective： To develop a new method to detect benzoic,sorbic,dehydroacetic acid in foods by capillary gas chromatography.Methods： The samples were deproteinized by NaOH-ZnSO4,defatted by benzine-ether firstly,then acidized with hydrochloric acid,extracted by ethyl acetate and finally determined by capillary gas chromatography.Results： The linear range was 50 μg^1000 μg（r0.9990）.When taken sample of 1.0 g,the lowest detecting limits of sorbic,dehydroacetic,benzoic were 0.033 g/kg,0.050 g/kg,0.010 g/kg.The recovery range was 90%~102%.RSD was 0.96%~3.3%.Conclusion： This method is stable,rapid and repeatable to detect the benzoic,sorbic,dehydroacetic acid of bulk foods.

关 键 词：山梨酸 脱氢乙酸 苯甲酸 气相色谱法 

分 类 号：O657.71[理学—分析化学]