番茄采后早疫病菌拮抗菌的筛选及发酵条件优化研究  

Screening of Antagonistic Bacteria for Alternaria solani and Optimizing of Its Fermentation Condition

作　　者：童志丹[1] 易有金[1,2,3] 杨建奎[4] 夏菠[1] 刘华金[1] 陈雪[1] 

机构地区：[1]湖南农业大学食品科学技术学院,湖南长沙410128 [2]湖南省食品科学与生物技术重点实验室,湖南长沙410128 [3]湖南省发酵工程技术中心,湖南长沙410128 [4]湖南农业大学理学院,湖南长沙410128

出　　处：《安徽农业科学》2011年第27期16645-16647,共3页Journal of Anhui Agricultural Sciences

基　　金：国家自然科学基金项目(31071738;31000827);湖南省教育厅重点项目(10A051);湖南省自然科学基金项目(09JJ-3032);湖南农业大学稳定人才科学基金项目(07WD18)

摘　　要：[目的]筛选番茄采后早疫病菌拮抗菌,并优化其发酵条件。[方法]从番茄果实中分离出15株菌株,通过平板对峙法从中筛选出对番茄早疫病有拮抗效果的菌株,并对其摇瓶发酵培养条件(pH、温度、培养时间、转速、装瓶量和接种量)进行了优化。[结果]从番茄果实中筛选出了对番茄采后早疫病菌具有明显拮抗效果的菌株004,其最佳发酵条件为:初始pH7.0,温度30℃,装液量100/250 ml,接种量2.0%,培养时间48 h,转速210 r/min。通过发酵条件优化,发酵滤液抗菌活性得以提高,抑菌圈从0.20 cm提高至0.70 cm。[结论]为防治番茄早疫病菌提供了参考。[Objective] The aim was to screen antagonistic bacteria for Alternaria solani and optimize their fermentation condition.[Method] 15 strains were isolated from tomato fruit,and then antagonistic bacteria strains for Alternaria solani were screened from them by plate method,furthermore fermentation conditions for the screened antagonistic bacteria strain were optimized by single factor experiment.[Result] An antagonistic bacteria 004 with strong antagonistic effect on Alternaria solani was screened out by plate method from tomato and branches.The optimal fermentation conditions for antagonistic bacteria 004 were as followed： the medium initial pH7.0,the fermentation temperature 30 ℃,the quantity of medium 100 ml in a 250 ml flask,the inoculation volume of 2%,the fermentation cycle of 48 h and the rotation speed of 210 r/min.The optimized inhibition zone（0.70 cm） was increased by 0.50 cm compared with that of the original conditions（0.2 cm）.[Conclusion] The research provides reference for controlling Alternaria solani.

关 键 词：番茄早疫病菌 拮抗菌 发酵条件 优化 

分 类 号：S436.412.14[农业科学—农业昆虫与害虫防治]