DEB-2菌株抗真菌活性物质对半夏组培生长的影响  

Effects of DEB-2 Antifungal Compound to Tissue Culture of Pinellia ternate

作　　者：吴璇[1,2] 康冀川[3,1] 雷帮星[1] 何劲[3] 杨秀超[2] 吴定基[2] 

机构地区：[1]贵州大学西南药用生物资源教育部工程研究中心,贵州贵阳550025 [2]贵州大学生命科学学院,贵州贵阳550025 [3]贵州大学贵州省农业生物工程重点实验室,贵州贵阳550025

出　　处：《贵州农业科学》2011年第9期114-117,共4页Guizhou Agricultural Sciences

基　　金：贵州省自然科学基金项目"石斛内生菌对植物病害及组培污染菌的生物防治研究"[黔科合J字(2008)2090];贵州省农业攻关项目"辣椒真菌病害生物防治及其田间配套关键技术"[黔科合NY字(2011)3034]

摘　　要：为了开发DEB-2菌株抗真菌活性物质成为组培抗真菌添加剂,采用生长速率法研究了DEB-2菌株抗真菌活性物质对12株组培常见污染菌的抑制作用,并将该抗真菌活性物质添加到组培培养基中,观察其对半夏组培苗生长的影响。结果表明,DEB-2菌株抗真菌物质对组培常见污染菌有较强的抑制作用,在20mL/L浓度下能明显抑制大多数常见组培污染真菌的生长,但对半夏的生长无显著影响;在30mL/L浓度下能显著抑制半夏不定芽的再生率;在40mL/L浓度下对不定芽生长高度的抑制作用极显著,对半夏的生长势有明显的抑制作用。In order to develop the DEB-2 antifungal compound to become antifungal additive of tissue,the inhibition activity to common contaminated fungi of tissue culture of DEB-2 antifungal compound and the effects of the growth of P.ternate were studied.The results showed that the antifungal compound of DEB-2 had strong inhibition activity to contaminated fungi of tissue culture.At the concentration of 20 mL/L,the antifungal compound could obviously restrain the growth of contaminated fungi,but had no effects to the growth of P.ternate.However,the antifungal compound could restrain the regeneration rate of P.ternate＇s adventitious buds at 30 mL/L of antifungal compound.The height of P.ternate＇s adventitious buds was strongly restrained at 40 mL/L of antifungal compound,but the growth vigor of P.ternate was not obviously inhibited.

关 键 词：DEB-2 抗真菌物质 真菌污染 组培 半夏 

分 类 号：S567.23[农业科学—中草药栽培]