壳聚糖固定化琼脂酶的研究  

Study on immobilization of agarase by chitosan

作　　者：朱启忠[1] 朱慧文[1] 孙延娜[1] 周晓龙[1] 郝梅[1] 郭振博[1] 刘丽丽[1] 

机构地区：[1]山东大学威海分校海洋学院,威海264209

出　　处：《生物加工过程》2011年第5期1-5,共5页Chinese Journal of Bioprocess Engineering

基　　金：国家创新性基金资助项目(101042239);山东大学科技立项重点项目专项基金资助项目(A10010)

摘　　要：采用壳聚糖微球对琼脂酶进行固定化,在单因素实验的基础上用正交试验法确定最佳固定化工艺。结果表明:在戊二醛体积分数为2.5%,交联时间为6 h,加酶量为15 mL,固定时间为3 h时固定酶的活力最高;固定化酶的最适反应温度及最适pH分别为50℃和8.5,高于游离酶;同时其热稳定性及操作稳定性均高于游离酶。Key factors for agarase immobilization on chitosan microspheres were investigated by the orthogonal experimental design. The results showed that the best efficiency of immobilization was obtained by using chitosan when the chitosan was cross linked with 2.5 % glutaraldehyde for 6 h, mixed three volume of free enzyme with one gram of carrier and reacted with agarase for 3 h. The optimum pH and the tem- perature of the immobilized enzyme were 8.5 and 50 ℃. The immobilized enzyme showed that better stability for pH and the temperature was higher than free enzyme.

关 键 词：琼脂酶 壳聚糖 固定化 

分 类 号：Q814.2[生物学—生物工程]