应用荧光原位杂交技术检测停育胚胎或绒毛染色体数目异常  

Fluorescence in Situ Hybridization to detect Fetal Chromosome Abnormalities in Missed Abortion

作　　者：张宁[1] 闫素文[1] 郝冬梅[1] 徐斌[1] 

机构地区：[1]解放军沈阳202医院全军计划生育优生优育技术研究所,辽宁沈阳110003

出　　处：《沈阳医学院学报》2011年第3期159-161,共3页Journal of Shenyang Medical College

摘　　要：目的:使用荧光原位杂交技术(fluorescence in situ hybridization,FISH)对停育胚胎染色体数目异常进行快速检测并评估其临床应用价值。方法:使用第13、18、21、X和Y 5条染色体特异性的FISH探针对66例停育胚胎绒毛进行分析。结果:13、18、21、X和Y数目异常20例,占46.97%,正常例数35例,间异例数11例。在所有异常胚胎中性染色体异常占75%,其中Turner's Syndrome 45X(TS 45X)所占比例最高与其他任何一种异常的发生率相比差异显著(P<0.05)。结论:FISH检测停育胚胎第13、18、21、X和Y数目异常是简单、快速和准确的诊断方法。Objective：To study the number abnormality of embryonic chromosomes in missed abortion by fluorescence in situ hybridization（FISH）,and its clinical application were evaluated.Methods： The uncultured villi from 66 women were performed FISH using specific DNA probes of 13,18,21,X and Y chromosomes.Results： 13,18,21,X and Y chromosomes showed number abnormal in 20 cases,accounting for 46.97%,normal in 35 cases,and abnormal amniocyte cell in 11 cases.75% of the abnormal embryos were neutral chromosomal abnormalities,in which Turner＇s Syndrome 45X（TS 45X） made up the highest percentage than any others,presented a significant difference in the incidence（P0.05）.Conclusion： FISH is an effective and reliable method in rapid diagnosis of chromosome number abnormality for missed abortion.

关 键 词：荧光原位杂交技术 产前诊断 染色体数目异常 

分 类 号：R714.153[医药卫生—妇产科学]