猪骨蛋白对骨质疏松大鼠尿中钙磷水平的影响  

Influence of pocine bone protein to the levels of calcium,phosphate and hydroxyproline in urine of the rats with osteoporosis

作　　者：张萌[1] 张陶然[1] 周俊飞[1] 安玉会[1] 

机构地区：[1]郑州大学基础医学院生物化学与分子生物学教研室,河南郑州450052

出　　处：《河南医学研究》2011年第3期261-263,共3页Henan Medical Research

摘　　要：目的:探讨猪骨蛋白对骨质疏松大鼠尿液中钙磷和羟脯氨酸水平的影响。方法:取3月龄Wistar大鼠60只,随机分成6组。A组大鼠灌胃2.5 ml/d生理盐水,其它各组大鼠2.5 ml/(kg.d)肌肉注射地塞米松,2次/周,共6周造成骨质疏松模型。之后,A和B组灌胃5ml/d生理盐水,C组灌胃300 mg/(kg.d)接骨七厘片,D,E,F组分别灌胃200 mg/(kg.d),100 mg/(kg.d),50 mg/(kg.d)猪骨蛋白。12周后取尿测尿钙和尿磷水平。结果:D和E组大鼠尿钙水平比生理盐水组降低(P<0.05);F组与生理盐水组比无显著性差异。结论:猪骨蛋白能降低骨质疏松模型大鼠尿钙水平。Objective：To explore the influence of porcine bone protein to calcium,phosphate and hydroxyoproline.Method：60 Wistar rats,which are about 3 monthes older,were divided into 6 groups.Exception of A group,which 2.5 ml/d of physiological saline was poured into rat,the rats in other groups were injected by dexamethasone,2.5 ml/（kg·d）,2 times per week,total 6 weeks,the rat model of osteoporosis to be formed.Then,the rats in A and B groups were poured by physiological saline,5 ml/d per rat.The rats in C groups were poured by 300 mg/（kg·d） of Jiegu Qili Pian.The rats in D,E,F groups were respectively poured into 200 mg/（kg·d）,100 mg/（kg·d） and 50 mg/（kg·d） of porine bone protein.After 12 weeks,The levels of calcium（Ca） and phosphate（P） in urine were detected.Results：Comparing with B group,the levels of Ca in urine in D and E groups were obviously lower（P0.05）.However,the levels of Ca in F groups had no difference.Conclusion：Porcine bone protein decreases the levels of Ca in the urine of rats with osteoporosis.

关 键 词：骨质疏松 大鼠模型 猪骨蛋白 尿钙 尿磷 尿羟脯氨酸 

分 类 号：R-332[医药卫生]