肝水解肽体外活性测定方法的探讨研究  

Research on the method for determining the bioactivity of liver hydrolysates in vitro

作　　者：王灿[1] 吴利红[1] 史芳亮[1] 邵泓[1] 陈钢[1] 

机构地区：[1]上海市食品药品检验所,上海201203

出　　处：《药物分析杂志》2011年第10期1982-1986,共5页Chinese Journal of Pharmaceutical Analysis

摘　　要：目的:建立肝水解肽体外生物学活性测定方法。方法:将不同浓度肝水解肽作用于正常人肝L02细胞,WST-8比色法测定L02细胞增殖情况。同时对实验条件,包括稀释液、细胞接种密度、药物作用时间等进行探讨,建立肝水解肽体外活性测定方法,并用该方法对2个厂家生产的2批产品进行活性检测。结果:获得了比较稳定可靠的实验参数:稀释液为RPMI-1640培养液,细胞接种密度为4.0×104个.mL-1,药物作用时间为48～72 h,肝水解肽浓度为0.5,0.25,0.12,0.06,0.03,0.015 mg.mL-1。由这些实验参数建立了肝水解肽活性测定方法。用该方法检测的2批产品活性均合格,重复实验3次,每次实验结果均一致,RSD均低于10%。结论:该方法可用于肝水解肽体外生物学活性测定。Objective：To establish the method for determining bioactivity of liver hydrolysates in vitro.Methods：Treated L02 cell with different concentration of liver hydrolysates,cell proliferation was determined by WST-8 colorimetric assay.Experiment conditions are explored,including dilution,cell density,cell culture time.Results：Stable and reliable method parameters were derived：RPMI-1640 culture is more suitable dilution,cell density was 4.0×104 mL^-1,culture time was 48-72 hours.As a result,we established the method for determining bioactivity of liver hydrolysates in vitro.Using the method,bioactivities of 2 bathches＇ products from different companies were determined 3 times,the results were consistent and the RSD was lower than 10%.Conclusion：The method was suitable for determining bioactivity of liver hydrolysates in vitro.

关 键 词：生化药物 肝水解肽 正常人肝细胞 WST-8比色法 生物学活性测定 

分 类 号：R917[医药卫生—药物分析学]