广西不同品种柑橘皮中橙皮苷含量测定  

Content analysis of hesperidin in Pericarpium Citri Retiulatae from different species in Guangxi

作　　者：韦媛媛[1] 陈忠坤[1] 肖芳[1] 周吴萍[1] 陈晓伟[1] 

机构地区：[1]广西工学院生物与化学工程系,柳州545006

出　　处：《食品科技》2011年第10期256-258,共3页Food Science and Technology

基　　金：广西教育厅科研项目(200911LX185).

摘　　要：目的：构建橙皮苷的含量测定方法,测定5种不同品种柑橘皮中橙皮苷含量。方法：采用正交实验法确定最佳提取条件。采用薄层-紫外法分离测定柑橘皮中的橙皮苷。结果：橙皮苷在2.7~27.0μg/mL（r=0.9992,n=6）范围内浓度与吸光度呈良好的线性关系。5种不同品种柑橘皮的橙皮苷含量在24.45～30.87mg/g之间。其中脐橙皮中橙皮苷含量最高。结论：该法测定柑橘皮中橙皮苷含量,对仪器要求不高,准确可靠,可用于柑橘皮质量控制。Objective：To establish a fast and simple determination method for hesperidin content from five different species of Pericarpium Citri Retiulatae in Guangxi.Methods：The optimum extracting condition was optimized through orthogonal design test,and the hesperidin were separated and determined by TLC-UV.Results：The calibration curve showed a good linear relationship when hesperidin was in 2.7～27.0 μg/mL（r=0.9992,n=6）.The hesperidin contents in the samples from different species were 24.45～30.87 mg/g.The results showed the hesperidin content in the sample from the peel of navel orange is highest.Conclusion：The method for the determination of hesperidin from Pericarpium Citri Reticulatae has high duplication and accuracy.It can be used for quality control of Pericarpium Citri Reticulatae.

关 键 词：柑橘皮 橙皮苷 含量测定 薄层色谱-紫外分光光度法 

分 类 号：R284.2[医药卫生—中药学]