样品前处理方法对食品中沙门菌PCR检测结果的影响  

Influence of sample pre-treatment on PCR results of salmonella in food

作　　者：肖建伟[1,2] 王兰芳[1] 沈利[1] 张蓉[2] 曹英川 

机构地区：[1]上海同济大学医学院,上海200092 [2]上海市杨浦区疾病预防控制中心,上海200090

出　　处：《上海预防医学》2011年第9期441-443,共3页Shanghai Journal of Preventive Medicine

摘　　要：[目的]探讨不同的标本前处理方法对食品中沙门菌DNA检测的影响。[方法]对沙门菌人工污染的各类食品增菌液,采用增菌液静止与混匀、增菌液沉淀清洗与不清洗的不同样本前处理方法,通过荧光定量PCR检测方法,比较不同前处理方法对食品中污染沙门菌DNA检测的影响。[结果]在熟食、水产品、生肉、奶制品的增菌液中,静止的方法比混匀的方法提取的DNA量要多,经荧光定量PCR检测,差异有统计学意义。对熟食、生肉、奶制品的增菌液,离心沉淀清洗一遍比不清洗一遍提取的DNA量要多,而水产品不受此种处理方法影响。[结论]对食品增菌液进行DNA提取时,应取上清液而不用混匀液,并应对增菌液的离心沉淀进行一次清洗,以达到更准确的检测结果。[Objective] To compare the different effects on DNA detection of two kinds of specimen pre-treatment methods in food salmonella detection. [Methods] We analyzed the influence of pre-treatment on salmonella DNA detection,using static and blending sampling method to deal with kinds of food.Meanwhile we detected the influence on salmonella DNA detection by cleaning or not cleaning Enrichment Broth centrifugal sedimentation. [Results] It was found to be more accurate for the result to use static method to deal with cooked food,aquatic products,raw,dairy than blending method,and the difference found in the result was statistically significant.In the comparison of the outcome from cleaning and not cleaning the food Enrichment Broth centrifugal sedimentation,it was found to be more accurate for the outcome to clean the Enrichment Broth centrifugal sedimentation of cooked food,raw meat,dairy,but aquatic products were not affected by the treatment. [Conclusion] We should use supernatant fluid instead of blending fluid,when conducting salmonella DNA detection of food Enrichment Broth,and clean the Enrichment Broth centrifugal sedimentation,in order to achieve more accurate PCR results.

关 键 词：样品前处理 食品 沙门菌 检测 

分 类 号：R155.5[医药卫生—营养与食品卫生学]