厚壳贻贝血细胞及血清抗菌肽的分析  

Analysis of Antimicrobial Peptides from Blood and Serum of Mytilus coruscus

作　　者：杨丽佳[1] 武梅[1] 王信超[1] 李楠楠[1] 杨林[1] 陈铭杰[1] 廖智[1] 

机构地区：[1]浙江海洋学院海洋科学学院海洋生物资源及分子工程实验室,浙江舟山316004

出　　处：《中国农学通报》2011年第23期58-64,共7页Chinese Agricultural Science Bulletin

基　　金：浙江省科技厅面上科研农业项目"贻贝抗菌功能多肽的提取与应用研究"(2009C32016);农业部海洋与河口渔业资源及生态重点开放实验室开放课题"厚壳贻贝抗菌肽的结构与功能研究"(开-09-10);2009年浙江省大学生科技创新活动(新苗人才计划)项目"厚壳贻贝抗菌肽的分子多样性分析"

摘　　要：厚壳贻贝(Mytilus coruscus)是中国东部海域广泛分布的具有重大经济价值的水产养殖贝类,其血淋巴中含有多种抗菌肽分子。为了解厚壳贻贝血细胞和血清抗菌肽分子的组成特点,采用多维高效液相色谱对厚壳贻贝血清及血细胞的抗菌肽进行分离纯化,从中鉴定了多种具有抗菌活性的抗菌肽分子,质谱鉴定结合cDNA文库数据分析表明厚壳贻贝血细胞和血清的抗菌肽主要属于Mytilin和Myticin抗菌肽家族,分子量在3000～7000Da之间,血细胞和血清抗菌肽在分子组成、抗菌谱方面均存在差异,此外还发现有分子量在10000Da左右的新型抗菌肽分子。以上工作为深入了解厚壳贻贝Mytilin抗菌肽的分子多样性、了解厚壳贻贝的免疫机制以及从中筛选具有开发价值的抗菌肽分子奠定了基础。Mytilus coruscus widely spreads all through the littoral extent of East China Sea with important economic value.Abundant peptides with antimicrobial activity were identified from hemocytes of mussel in previously works.To elucidate the compositive characterization of antimicrobial peptides from blood cell and serum of Mytilus coruscus hemocytes,high performance liquid chromatogram were used and several fractions with distinct antimicrobial activity were isolated from blood cell and serum of Mytilus coruscus,respectively.Mass spectrometry combined cDNA data analysis were performed to characterize the molecular mass and annotation of some peptides.Some of antimicrobial peptides identified in this work were considered belong to Mytilins or Myticins family with molecular mass ranged from 3000 to 7000 Da.The difference between blood cell and serum antimicrobial peptides mainly exists in molecular composition and antimicrobial spectrum.In addition,a novel peptide with strong antimicrobial activity was found in serum of Mytilus coruscus and the molecular mass of this peptide is more than 10000 Da.The results of this work build the foundation for further research on the molecular diversity of antimicrobial peptides,immune mechanisms of Mytilus coruscus,and screening for new peptides with applicable value.

关 键 词：厚壳贻贝 抗菌肽 血细胞 血清 质谱 

分 类 号：Q51[生物学—生物化学]