EGCG诱导前列腺癌细胞PC-3凋亡及对Survivin蛋白表达的影响  

EGCG Promoting Apoptosis of Human Prostate Cancer Cell PC-3 and Its Influence on Expressions of Survivin Protein

作　　者：高漓[1] 张天禹[1] 尤剑鹏[1] 赵超群[1] 谭宁[2] 黄岚珍[2] 

机构地区：[1]桂林医学院附属医院泌尿外科,桂林541001 [2]桂林医学院科学实验中心,桂林541001

出　　处：《华中科技大学学报（医学版）》2011年第5期580-584,共5页Acta Medicinae Universitatis Scientiae et Technologiae Huazhong

基　　金：广西壮族自治区卫生厅医疗卫生重点科研课题(No.重200982);广西壮族自治区卫生厅科研课题(No.Z2007320);科技部癌基因及相关基因国家重点实验室开放课题(No.90-08-01)

摘　　要：目的探讨表没食子儿茶素没食子酸酯(epigallocat-echin-3-gallate,EGCG)对人前列腺癌细胞PC-3凋亡及对其Survivin蛋白表达的影响。方法使用不同浓度的EGCG(0、20、40、80μg/mL)处理前列腺癌细胞株PC-3,通过细胞增殖曲线实验检测EGCG对PC-3细胞增殖的影响;流式细胞仪检测不同浓度EGCG处理PC-3细胞48h后对细胞周期及凋亡的影响;用RT-PCR法检测各浓度EGCG作用48h后PC-3细胞中Survivin基因的表达差异,使用Western blot进行确认。结果 EGCG可以抑制PC-3细胞的增殖并诱导其凋亡,其作用随EGCG浓度的增加而增强(P<0.05);Sur-vivin在PC-3细胞中高表达,EGCG促使PC-3细胞的Survivin表达下调,其表达水平随EGCG浓度的增加而减少(P<0.01)。结论 EGCG能下调PC-3细胞中Survivin蛋白的表达,这可能是EGCG抑制PC-3细胞增殖、诱导其凋亡的关键机制。Objective To investigate the apoptosis in human prostate cancer cell line PC-3 induced by different concentrations of epigallocat-echin-3-gallate（EGCG）and to study the influence of EGCG on the expressions of Survivin protein. MethodsPC-3 cells were cultured in vitro,and incubated with different concentrations of EGCG（0,20,40 and 80 μg/mL）,respectively.The cell proliferation was measured by growth curve.The apoptosis of PC-3 cells was tested by flow cytometry.The expression of Survivin was detected by using reverse transcription-polymerase chain reaction（RT-PCR）assay,and conformed by Western blot. Results EGCG inhibited the proliferation of PC-3 cells in a time-and concentration-dependent manner.The apoptosis of PC-3 cells was induced by EGCG,and apoptotic rate was increased with the increased EGCG concentration.EGCG could promote the expression of Survivin in PC-3 cells in a concentration-dependent fashion. Conclusion EGCG can inhibit the growth of PC-3 cells and induce the apoptosis,which may be principally associated with the down-regulation of Survivin.

关 键 词：表没食子儿茶素没食子酸酯 前列腺癌 SURVIVIN蛋白 细胞增殖 细胞凋亡 

分 类 号：R736.1[医药卫生—肿瘤]