耐高温植酸酶毕赤酵母工程菌发酵中试条件优化  

Optimization of Pilot-Scale Conditions of Thermostable Phytase Fermentation by Engineered Pichia pastoris Strain

作　　者：黄魁英 夏枫耿 黄乐天 明飞平 杜少平 

机构地区：[1]广州市微生物研究所,广东广州510663

出　　处：《现代食品科技》2011年第10期1238-1241,共4页Modern Food Science and Technology

摘　　要：本文对植酸酶毕赤酵母基因工程菌PEY-2的发酵条件进行研究,结果表明:其摇瓶最佳产酶条件为诱导时间72 h,诱导前适宜增殖时间48 h,接种量10%,种龄24 h,诱导初始pH 6.0,生长阶段初始pH 5.5。在此基础上进行了50 L罐的发酵中试,50 L罐诱导产酶量达5.0×103 IU/mL,实现了高密度发酵。热稳定性试验表明:植酸酶液pH 2.5,90℃热处理20 min残留酶活69%,pH 5.5,90℃热处理20 min残留酶活75%,热稳定性良好,可以满足饲料高温制粒的要求。The phytase fermentation conditions of the genetic-engineered Pichia pastoris strain PEY-2 were studies in this paper. The optimal phytase fermentation conditions were： induction time 72 h, enrichment time before induction 48 h, inoculum size 10%, seed age 24 h, pH for cell growth and inducement at 5.5 and 6.0 respectively. In scale-up culture in 50 L fermenter, phytase fermentation of high density is achieved and the activity of phytase highly reached 5.0× 103 IU/mL by constant dissolve oxygen fed-batch way of glucose. Thermal stability tests showed that after 20-minute incubation at 90 ℃, the remaining activity of the phytase was 69% at pH 2.5 and 75% at pH 5.5 respectively,. The phytase was thermostable and could meet requirements of feed processing.

关 键 词：植酸酶 毕赤酵母 发酵中试 优化 热稳定性 

分 类 号：TQ925[轻工技术与工程—发酵工程]