皮氏罗尔斯通氏菌株DX-T3-01的耐镉性能及镉富集机理  

Cadmium Tolerance and Bio-accumulation Mechanisms of Ralstonia pickettii strain DX-T3-01

作　　者：付瑾[1] 谢学辉[1] 钱林[1] 柳建设[1] 

机构地区：[1]东华大学环境科学与工程学院,上海201620

出　　处：《应用与环境生物学报》2011年第5期717-721,共5页Chinese Journal of Applied and Environmental Biology

基　　金：全国优秀博士学位论文作者专项资金项目(No.200549);国家自然科学基金项目(No.50874032);上海市重点学科建设项目(No.B604)资助~~

摘　　要：从江西德兴铜矿土壤中分离筛选到一株具有较强耐镉(Cd)能力的细菌DX-T3-01,经生理生态及16S rRNA分析进行鉴定,研究其对不同浓度Cd2+的耐受特性及对重金属Zn2+、Cu2+、Ni2+的耐受性,进一步利用扫描电镜-能谱仪(SEM-EDX)和红外光谱(FTIR)探讨菌株对Cd2+累积去除的机理.经鉴定,耐镉细菌DX-T3-01为皮氏罗尔斯通氏菌(Ralstonia pickettii);Cd2+最高耐受浓度(MTC)为16 mmol L-1,最低抑制浓度(MIC)为4 mmol L-1,并可耐受一定浓度的Zn2+、Cu2+;菌体积累Cd2+后细胞表面有沉淀物附着并有镉元素检出,对Cd2+的累积主要依靠细胞壁上—PO43-、胺基中的—C—N—、—M—O(O—M—O)、C=O和酰胺基(—CO—NH—)基团.A cadmium-resisting bacterium,strain DX-T3-01,was isolated from the tailing dam of Dexing Copper Mine in Jiangx,China,and its tolerance to cadmium was investigated in liquid medium with different Cd2＋ concentrations.Besides,the resistance of the strain to other heavy metals such as Zn2＋,Cu2＋ and Ni2＋ were also studied.The mechanism of cadmium accumulation by stain DX-T3-01 was evaluated by scanning electron microscopy-energy dispersive X-ray spectroscopy（SEM-EDX） and infrared spectroscopy（FTIR）.The strain was identified as Ralstonia pickettii according to biochemical characteristics and 16S rRNA sequences analysis.The results of resistance experiments showed that the maximum tolerated concentration（MTC） and minimal inhibitroy concentration（MIC） of cadmuim were 16 mmol L-1and 4 mmol L-1,respectively.The results of machanism experiments indicated that much granular cadmium was precipitated on the cell wall of bacteria based on the possible functional groups such as —PO43-,—C—N—,—M—O（O—M—O）,C=O and —CO—NH—.Fig 5,Ref 16

关 键 词：皮氏罗尔斯通氏菌 菌株筛选 16SrRNA 耐镉特性 红外光谱 

分 类 号：X172[环境科学与工程—环境科学] R53[医药卫生—内科学]