抑制c-myc基因表达对HL-60细胞表面分化抗原CD13、CD33的影响  

The effects of repressing c-myc expression in the HL-60 cells on CD13 and CD33

作　　者：陈龙[1] 王湘辉[1] 陈洁平[2] 

机构地区：[1]兰州军区兰州总医院肿瘤科,甘肃兰州730050 [2]第三军医大学西南医院血液科,重庆400038

出　　处：《现代肿瘤医学》2011年第10期1940-1942,共3页Journal of Modern Oncology

基　　金：国家"863"计划资助项目(编号:Z20-01-02)

摘　　要：目的:利用重组反义c-myc腺病毒载体(Ad-AS-c-myc)抑制HL-60细胞c-myc基因表达,检测细胞表面分化抗原CD13、CD33变化,了解抑制HL-60细胞c-myc表达,对细胞分化及分化抗原的影响。方法:重组Ad-AS-c-myc病毒按感染强度(MOI)为100的转染剂量,转染HL-60细胞。RT-PCR检测c-myc表达。流式细胞仪检测CD13、CD33阳性细胞率。同时对转染细胞行Wright's染色,观察细胞形态变化。结果:Ad-AS-c-myc转染HL-60细胞后,c-myc基因表达降低,CD13阳性细胞率升高,CD33阳性细胞率降低。结论:抑制c-myc基因表达,使HL-60细胞CD13表达增强,CD33表达降低。细胞形态向成熟粒细胞方向分化。Objective： Using recombinant antisense c-myc adenovirus（Ad-AS-c-myc） to repress c-myc expression in HL-60 cells,detect change of CD13 and CD33,and to evaluate the differentiation by repressing c-myc expression in HL-60 cells.Methods： The HL-60 cells were transfected by recombinant adenovirus encoding antisense c-myc（MOI：100）.The transcription of c-myc was assessed by RT-PCR.The expression of CD13 and CD33 was determined by flow cytometry in HL-60 cells.The morphology of the transfected HL-60 cells observated by wright＇s staining.Results： The level of c-myc transcription and the version could been strongly inhibited by Ad-AS-c-myc in the transfected HL-60 cells.The rate of CD13＋ was increased,CD33＋ reduced.Conclusion： Repressing c-myc expression in HL-60 cells,changed the rates of CD13＋ and CD33＋.The morphology of transfected HL-60 cells tend to matured granulocytic cell.

关 键 词：重组反义c-myc腺病毒 CD13 CD33 C-MYC基因 分化 

分 类 号：R730[医药卫生—肿瘤] R733.7[医药卫生—临床医学]