甘蓝型油菜隐性上位互作核不育基因(Ms1)精细定位  

Fine Mapping of a Recessive Genic Male Sterility Gene (Ms1) in Rapeseed (Brassica napus)

作　　者：江莹芬[1] 陈凤祥[1] 李强生[1] 胡宝成[1] 吴新杰[1] 侯树敏[1] 范志雄[1] 费维新[1] 荣松柏[1] 

机构地区：[1]安徽省农业科学院作物研究所,安徽省农作物品质改良重点实验室,合肥230031

出　　处：《分子植物育种》2011年第5期599-604,共6页Molecular Plant Breeding

基　　金：国家863计划(2009AA101105;011AA10A104);安徽省农科院作物所青年创新基金;安徽省自然科学基金(11040606M83);安徽省十一五科技攻关项目(06003011B)共同资助

摘　　要：甘蓝型油菜细胞核雄性不育是杂种优势利用的重要途径。隐性上位互作核不育系9012A已经广泛用于杂交种子生产,其不育性受两对隐性重叠不育基因(ms1和ms2)与一对隐性上位抑制基因(rf)互作控制。ms1和ms2同时纯合(ms1ms1ms2ms2)表现不育,但隐性纯合rf(rfrf)对ms1ms1ms2ms2的表达起抑制作用,又可使其表现可育(TAM系,ms1ms1ms2ms2rfrf)。本研究利用AFLP和SRAP分子标记技术,分别筛选了884对AFLP引物和506对SRAP引物,筛选到了14个与不育基因Ms1基因紧密连锁的标记,其中4个标记与育性共分离。Ke等发表的AFLP标记E-ACA/P-CTG在本研究群体中与Ms1基因相距0.1cM。紧密连锁标记测序序列BLASTN结果表明,其和拟南芥第五染色体高度同源,并且通过和已测序的甘蓝C基因组序列进行比对,将其定位在C9染色体的末端。本研究结果有助于该基因的分子标记辅助选择和克隆。本文还对前人发表的与Ms1基因连锁的标记在本研究群体中的分离情况作了探讨。Genic male sterility is one of the most valuable resources for heterosis utilization in Brassica napus. The recessive epistatic genic male sterile line 9012A has been playing an increasing role in hybrid cultivar development in China. That sterility is controlled by two pairs of recessive duplicated sterile genes（ms1 and ms2） and one pair of recessive epistatic inhibitor gene （rf）. Homozygosity at the rf locus （rfrf） inhibits the expression of the two recessive malt sterility genes in homozygous ms1ms1ms2ms2 plants and results in a male fertile phenotype （TAM line, ms1ms1ms2ms2rfrf）. In this study, 884 pairs of AFLP and 506 pairs of SRAP maker system were used for investigating the genotype of Ms1ms1ms2ms2rfrf and ms1ms1ms2ms2rfrf segregated in a NIL population of 304 individuals. As a result, we obtained 14 makers tightly linked with the Ms1 gene, of which 4 markers co-segregated. The AFLP marker E-ACA/P-CTG reported by Ke et al. has a genetic distance of 0.1 cM in our research population. BLAST analysis with sequences of tightly linked makers shows that, some of the sequences have high similarities with those genomic DNA sequences from Arabidopsis chromosome 5. Most notably, these sequences generated from the most tightly linked markers with Ms1 gene were perfectly anchored to one of the terminal of chromosome C9 of Brassica olerecea. All of these results of this study will benefit for map-based cloning and maker assistant selection of Ms1 gene. Additionally, the segregation of the markers link with Ms1 gene which has been reported by other authors was investigated and compared in our segregated population in this paper.

关 键 词：隐性上位互作核不育 细胞核雄性不育 不育基因 AFLP SRAP 

分 类 号：S565.4[农业科学—作物学]