整合素β3与肺癌A549细胞对ADM敏感性的关系  

Study of integrin β3 in sensitivity to chemotherapy of pulmonary adenocarcinoma A549

作　　者：栾巍[1] 赵立强[1] 

机构地区：[1]解放军第253医院呼吸内科,呼和浩特010051

出　　处：《临床肿瘤学杂志》2011年第9期777-779,共3页Chinese Clinical Oncology

摘　　要：目的探讨整合素β3(Integrinβ3)对人肺腺癌A549细胞系的药物敏感性的作用及可能机制。方法采用三维培养方法获得A549细胞团簇(MCS);血球计数器计数法比较阻断Integrinβ3作用前后A549 MCS对阿霉素(ADM)的敏感性;流式细胞术检测Integrinβ3表达和A549细胞凋亡情况。结果经过5天细胞培养,成功建立了A549 MCS。在一定浓度范围内(<0.02μg/ml),A549 MCS中Integrinβ3表达量随ADM浓度的增加而增加。阻断Integrinβ3的作用后,A549 MCS对ADM的敏感性增强,半数抑制浓度IC50由0.19μg/ml下降至0.11μg/ml,细胞凋亡率从(15.81±1.87)%上调至(30.14±2.89)%。结论 Integrinβ3的表达水平能够影响A549 MCS的药物敏感性,可能与Integrinβ3抑制凋亡相关。Objective To investigate the effect of integrin β3 on influencing the sensitivity to chemotherapy of human pulmonary adenocarcinoma A549 cells.Methods Human pulmonary adenocarcinoma A549 multicellular spheroids（MCS） were constructed with three dimensional cell culture methods.Cell counting using blood cell counter was employed to detect the sensitivity to ADM of A549 MCS before and after blocking integrin β3.The integrin β3 expression of A549 MCS and cell apoptosis was detected by flow cytometry.Results A549 MCS were successfully established.The integrin β3 expression of A549 MCS elevated with the concentration of ADM（0.02μg/ml）.Blocking integrin β3 leaded to higher sensitivity to ADM,and IC50decreased from 0.19μg/ml to 0.11μg/ml,and apoptosis rate increased from（15.81±1.87）% to（30.14 ± 2.89）%.Conclusion The cell adhesion molecules integrin β3 could influence the sensitivity to chemotherapy of A549 MCS and inhibiting cell apoptosis might be its mechanism.

关 键 词：人肺腺癌细胞系A549 INTEGRIN β3 凋亡 化学治疗 

分 类 号：R734.2[医药卫生—肿瘤]