HIF-lαasODN转染的树突状细胞对胶质瘤细胞生长的影响  

Effects of dendritic cell transduced by antisense oligodeoxynucleotides targeting hypoxia inducible factor- 1a gene on human glioma cells

作　　者：范月超[1] 冯力[1] 陈晨[1] 李中林[1] 谢满意[1] 苗发安[1] 

机构地区：[1]徐州医学院附属医院神经外科,江苏徐州221002

出　　处：《徐州医学院学报》2011年第10期654-657,共4页Acta Academiae Medicinae Xuzhou

基　　金：基金项目：徐州市科技局科技计划项目（XM07C074）

摘　　要：目的研究缺氧诱导因子-1a（HIF-1a）反义寡核苷酸（asODN）转染的树突状细胞（DC）与U251胶质瘤细胞融合后的抗胶质瘤活性。方法将HIF-1a asODN利用脂质体包裹转染DC，采用PEG化学融合方法将DC与U251胶质瘤细胞融合，通过MTT法及流式细胞仪检测胶质瘤细胞凋亡率。结果HIF-1a asODN转染DC组与各对照组相比细胞增殖下降，凋亡增加（P〈0．01）。结论HIF-1a asODN转染DC后，再与U251胶质瘤细胞融合能够比单纯使用HIF-1a asODN转染U251细胞或单纯使用DC细胞取得更好的抑制胶质瘤细胞生长及促进其凋亡的效果。Objective To antisense oligodeoxynucleotides （asODN） targeting hypoxia inducible factor - 1 a gene into dendritic cells （DC） , then fuse with glioma cell line U251 and to observe its effect of apoptosis inducing and growth restraining to U251 cells. Method The asODN targeting hypoxia inducible factor - 1a gene was transduced into DC by liposomes mediated transfection. Then the transduced DCs are fused with glioma cells. The growth of U251 cell was detected by 3 - （4,5 - dimethylthiazol - 2 - yl） - 2,5 - diphenyltetrazolium bromide （MTT） method. The change of cells apoptosis rate was measured by flow cytometry . Results MTT method revealed that the cell growth in the transfected group was inhibited most significantly （P 〈 0.01 ）. Flow cytometry analysis revealed glioma cell line U251 suffered a more notable apoptosis than other group （ P 〈 0. 01 ）. Conclusion After transduction of HIF - 1 a asODN into DC, the transduced DC can inhibited human glioma cell line U251 in vitro remarkably and the apoptosis of U251 cell can be induced significantly.

关 键 词：树突状细胞 胶质瘤细胞 缺氧诱导因子-1a 反义寡核苷酸 

分 类 号：R739.4[医药卫生—肿瘤]