高产β-葡聚糖酶菌株初步筛选与鉴定  

Preliminary Screening and Identification of High-yield Strains for β-glucanase

作　　者：邵金华[1] 朱智勇[1] 刘欢[1] 邓佳[1] 欧阳青[1] 李军艳[1] 

机构地区：[1]湖南科技学院,湖南永州425100

出　　处：《湖南农业科学》2011年第10期13-15,共3页Hunan Agricultural Sciences

基　　金：湖南省永州市市级课题(20110606)

摘　　要：利用β-葡聚糖酶能够降解含刚果红染料的高分子量大麦β-葡聚糖从而使培养基产生透明圈的特点,对采集的土样进行了分离纯化,得到一株高产β-葡聚糖酶的菌株并做了初步鉴定。结果表明,筛选到1株高产β-葡聚糖酶的菌株,命名为ZJ2132,其酶活可达142.36 U/mL。通过初步鉴定,该菌株为木霉属中的Trichoderma sp.。Abstract： Using the characteristics that β-glucanase can degrade high molecular weight barley β-glucan which containing Congo red dye to produce transparent circle to separate and purify the collected soil samples, and a high-yield strain for β-glucanase was obtained and identified preliminarily. The results showed that the screened high-yield strain for β-glucanase was named as ZJ2132, and its enzymatic activity can reach 142.36 U/mL. Through the preliminary identification, the ZJ2132 belong to Trichoderma sp. from Trichoderma.

关 键 词：Β-葡聚糖酶 菌株 筛选 鉴定 

分 类 号：Q934[生物学—微生物学]