开管型3,5-二氯苯基氨基甲酸酯纤维素衍生物键合手性毛细管电色谱柱的制备及应用  

Preparation and application of open tubular capillary column containing covalently bound cellulose,tris(3,5-dichlorophenylcarbamate) as chiral selector

作　　者：杨清清[1] 伍蓉 赵敬丹[3] 狄斌[3,4] 苏梦翔[3] 吴夏冰[3] 强淑萍[3] 樊菲[3] 

机构地区：[1]江苏省食品药品检验所,南京210008 [2]重庆市万州药品检验所,重庆404000 [3]中国药科大学药物分析教研室,南京210009 [4]药物质量与安全预警教育部重点实验室,南京210009

出　　处：《药物分析杂志》2011年第11期2108-2113,共6页Chinese Journal of Pharmaceutical Analysis

摘　　要：目的:制备键合型开管毛细管电色谱柱,用于手性药物分析。方法:合成了带有十一碳烯酰基的纤维素-三(3,5-二氯苯基氨基甲酸酯)衍生物,通过自由基聚合反应,将其键合于毛细管内壁,得到毛细管电色谱柱,并对手性药物进行色谱分离。结果:吡喹酮得到较好分离。结论:制备的毛细管柱对吡喹酮具有较好的识别能力,且柱效较高,对后续键合型开管毛细管电色谱柱的研究奠定了基础。Objective： To prepare immobilized cellulose tris（ 3,5 - dichlorophenylcarbamate） as chiral stationary phase on capillary column and study its properties on chiral drugs. Methods： Cellulose tris（3,5 -dichlorophenylcarbamate） derivatives having a small amount of 10 -undecenoyl groups were synthesized by a simple process and efficiently immobilized on capillary column by radical polymerization reaction with azobisisobutyronitrile （AIBN）as the initiator. The column was evaluated by chiral drugs. Results： D, L - praziquantel acquired baseline separation. Conclusion： Praziquantel got chiral recognition on the column, and obtained high plate number, which laid a foundation for further investigation of open tubular capillary column.

关 键 词：纤维素 键合 对映体 自由基聚合 电色谱 手性拆分 手性固定相 吡喹酮 

分 类 号：R917[医药卫生—药物分析学]