油菜蜂花粉中抑菌活性成分的分离纯化和结构鉴定  

Separation,Purification and Identification of Antibacterial Components in Rape Bee Pollen

作　　者：孙丽萍[1] 杜夏[1] 陈有根[2] 徐响[1] 安仲姚[3] 李春艳[3] 

机构地区：[1]中国农业科学院蜜蜂研究所,北京100093 [2]北京市药品检验所,北京100035 [3]东北农业大学资源与环境学院,黑龙江哈尔滨150030

出　　处：《食品科学》2011年第19期75-78,共4页Food Science

基　　金："十二五"国家科技支撑计划项目(2011BAD33B04);国家现代农业(蜂)产业技术体系建设专项(CARS-45-KXJ18);科技部农业科技成果转化资金项目(2009GB23260456)

摘　　要：以对枯草芽孢杆菌、大肠埃希氏菌、金黄色葡萄球菌、铜绿假单胞菌的抑菌能力为活性跟踪指标,以最小抑菌质量浓度(MIC)和最小杀菌质量浓度(MBC)为评价指标,从油菜蜂花粉水提物中分离得1个化合物,用波谱学进行结构解析,鉴定为山奈酚,其抑菌效果显著,是油菜蜂花粉抑菌活性成分,可作为植物源抗菌剂,提高油菜蜂花粉的应用价值。The bioassay-guided fractionation was used to isolate antibacterial components from aqueous extract of rape bee pollen.The MICs and MBCs of antibacterial components isolated against Bacillus subtilis,Escherichia coli,Staphylococcus aureus rosenbach and Pseudomonas aeruginosa were determined.An active component with strong antibacterial activity was obtained and identified as kaempferol by ESI-MS and NMR spectroscopy.Therefore,rape bee pollen is a natural antibacterial plant source and has promising application potential.

关 键 词：油菜蜂花粉 抑菌活性成分 结构鉴定 

分 类 号：S896.4[农业科学—特种经济动物饲养]