肉豆蔻提取物对IFN-γ干预的小胶质细胞BV2的调控作用  

Effects of nutmeg extraction on microglial BV2 cells treated with IFN-γ

作　　者：文现宇[1] 毛翘[1] 崔春爱[1] 

机构地区：[1]延边大学基础医学院解剖学教研部,吉林延吉133002

出　　处：《山东大学学报（医学版）》2011年第9期21-23,共3页Journal of Shandong University:Health Sciences

基　　金：吉林省教育厅科研基金资助项目[吉教科合字(2011)第4号]

摘　　要：目的探讨肉豆蔻提取物对鼠性小胶质BV2细胞的作用机制。方法实验分为对照组、IFN-γ模型组、肉豆蔻提取物复合处理组。采用WST-8试剂盒,观察肉豆蔻提取物对体外培养的鼠性小胶质细胞BV2生存率的影响,采用分子生物学技术,检测IFN-γ所诱导的诱导型一氧化氮合酶(iNOS)和环氧合酶2(COX-2)的表达水平。结果肉豆蔻提取物对鼠性小胶质细胞BV2无毒性,且抑制了IFN-γ所诱导的iNOS和COX-2的表达,与IFN-γ模型组比较差异显著(P<0.01)。结论肉豆蔻提取物对鼠性小胶质细胞BV2具有很好的抗神经毒性及抗炎作用。Objective To study the effect of nutmeg ethanol extract on murine microglial BV2 cells.Methods Murine microglial BV2 cells were divided into the control group,the IFN-γ model group,and the combined nutmeg ethanol extract treatment group.The WST-8 kit was used to detect the effect of nutmeg ethanol extract on murine microglial BV2 cell viability.The effects of interferon-γ（IFN-γ） and nutmeg ethanol extract on protein expressions of inducible nitric oxide synthase（iNOS） and cyclooxygenase 2（COX-2） in vitro were measured by Western blot.Results Nutmeg ethanol extract treatment did not affect cell viability as determined by the WST-8 cell counting kit.Western blot revealed that nutmeg ethanol extract could inhibit protein expressions of iNOS and COX-2 induced by IFN-γ.There were significant differences between the combined nutmeg ethanol extract treatment group and the IFN-γmodel group（P0.01）.Conclusion Nutmeg ethanol extract could exert anti-neurotoxicity and anti-inflammatory effects on murine microglial BV2 cells.

关 键 词：肉豆蔻 干扰素Ⅱ型 小胶质细胞 

分 类 号：R745.49[医药卫生—神经病学与精神病学]