中药对黄曲霉毒素B1中毒雏鸭肾组织抗氧化功能的影响  

Effects of Traditional Chinese Medicine on Antioxidant Function of Kidney Tissue in Poisoning Ducklings Induced by Aflatoxin B1

作　　者：石达友[1] 廖申权[2] 林远超[1] 刘念[1] 李鹏飞[1] 郭铭生[1] 唐兆新[1] 

机构地区：[1]华南农业大学兽医学院,广东广州510642 [2]广东省农业科学院兽医研究所,广东广州510640

出　　处：《中国家禽》2011年第21期26-29,共4页China Poultry

基　　金：广东省农业攻关项目(2008B20700004)

摘　　要：为观察黄曲霉毒素B(1Aflatoxin B1,AFB1)对雏鸭肾组织抗氧化功能的影响及复方中药对AFB1的颉颃效应,试验将90只7日龄雏鸭分为3组,每组30只。第Ⅰ组设为空白对照组,灌胃同等量二甲基亚砜;第Ⅱ、Ⅲ组为试验组,每天分别按0.1mg/kg剂量给第Ⅱ、Ⅲ组雏鸭灌胃AFB1一次,连续投药21d,试验期间给第Ⅲ组雏鸭日粮中添加2%复方中药。分别在给雏鸭投药后7、14、21d,检测雏鸭肾组织总抗氧化能力(T-AOC)、超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、谷胱甘肽过氧化物酶(GSH-Px)、谷胱甘肽还原酶(GR)及丙二醛(MDA)等抗氧化指标。试验结果显示,第Ⅱ、Ⅲ组雏鸭肾组织SOD、CAT、GSH-Px及GR活性与T-AOC均显著低于第Ⅰ组(P<0.05),而MDA显著高于第Ⅰ组(P<0.05);与第Ⅱ组相比,在日粮中添加复方中药的第Ⅲ组雏鸭肾组织各项抗氧化指标均得到明显的改善(P<0.05)。结果表明,黄曲霉毒素B1导致雏鸭肾组织抗氧化功能发生显著的变化,而复方中药能明显改善其变化。In order to study the influences of aflatoxin B1 （AFB1）on antioxidant function of kidney tissue in ducklings and the antagonistic effect of compound traditional Chinese medicine （TCM）on AFB1, seven-day-age ducklings were used as experimental animals and divided into three groups （30 animals each）. The group I was only administered in the same volume of DMSO with the other group as control. Group II , Ⅲ were administered intragastrically in AFB1 at a dose of 0.1 mg/kg body weight respectively, and group III was fed on mixed diet added with 2% TCM. All treat- ments were given once daily during 7 to 28 days age. Total-antioxidant capacity （T-AOC）,superoxide dismutase （SOD）, catalase （CAT）, glutathione peroxidase （GSH-Px）,glutathione reductase （GR）and malondialdehyde （MDA）of kidney tissue were detected on 7th, 14th,2hh day after treatment. The results showed that the activities of SOD,CAT, GSH-Px, GR and T-AOC significantly decreased in ducklings kidney tissue of group II ,III compared with group I （P〈0.05）, and the level of MDA significantly increased（P〈0.05）. There was a significant improvements in antioxidant function of kidney tissue of TCM treated group m compared with group II （P〈0.05）. The results revealed that TCM could ameliorate antioxidant function changes of kidney tissue induced by aflatoxin B1 in ducklings.

关 键 词：黄曲霉毒素B1 抗氧化功能 雏鸭 中药 

分 类 号：S831.5[农业科学—畜牧学]