出口肉及制品中盐酸克伦特罗的ELISA检测方法的建立  

Application of ELISA on residue determination of clenbuterol hydrochloride in export meat and products

作　　者：郑海松[1] 李云飞[1] 杨小娇[2] 余晓峰[1] 陈雪娇[1] 孙娟娟[1] 李刚[1] 

机构地区：[1]安徽出入境检验检疫局,合肥230022 [2]合肥工业大学生物与食品工程学院,合肥230009

出　　处：《食品科技》2011年第11期120-123,共4页Food Science and Technology

基　　金：国家标准项目(20073670-T-424);出入境检验检疫行业标准项目(2009B165)

摘　　要：介绍了酶联免疫吸附法测定出口肉及制品中的盐酸克伦特罗的方法。利用盐酸克伦特罗试剂盒对出口肉及制品中残留的盐酸克伦特罗用酶标仪进行测定分析,结果显示,盐酸克伦特罗的交叉反应为100%,猪肉罐头和牛肉中的检测限(LOD)为0.026(μg/kg)和0.028(μg/kg),样品的平均回收率在83.6%～91.3%之间,相对标准偏差为3.5%～5.6%,经HPLC确证假阳性率≤2.0%。表明酶联免疫分析定量法可快速、准确实现对食品中盐酸克伦特罗残留量的快速筛选。The method of ELISA applied in residue determination of clenbuterol hydroehloride in export meat and products was introduced in this paper. Use clenobuterol hydrochloride kit on export meat and products residual clenobuterol hydrochloride were determined by enzyme standard instrument. The results showed that the cross reaction of clenobuterol hydrochloride is 100%, the detection limit of canned beef and pork（LOD） is 0.026 ug/kg and 0.028 ug/kg, the average recovery of samples is between 83.6% and 91.3%, and relative standard deviation is 3.5%-5.6%, false positives confirmed by HPLC using 2.0%, by using HPLC corroborate false-positive rate not more than 2.0%. The results indicated that enzymelinked immunosorbent analysis quantitative method can realize rapid and accurate in food clenbuterol hydroehloride residues screening.

关 键 词：ELISA 盐酸克伦特罗 食品 快速检测 

分 类 号：TS207.53[轻工技术与工程—食品科学]