职业电磁辐射环境对大鼠小脑普肯耶细胞GluR2定位表达的影响  

Changes of GluR2 Protein in Cerebellar Purkinje Cell of Rats after Occupational Electromagnetic Irradiation

作　　者：刘勇[1] 黄尉初[1] 周文[2] 刘大星[1] 王修德[1] 

机构地区：[1]济南军区疾病预防控制中心,250014 [2]第三军医大学大坪医院野战外科研究所,400042

出　　处：《医学研究杂志》2011年第10期104-106,共3页Journal of Medical Research

摘　　要：目的探讨职业电磁辐射环境对大鼠小脑普肯耶细胞谷氨酸受体2(GluR2)及其磷酸化蛋白质定位表达的影响特点和规律。方法 Wistar大鼠给予平均功率密度为5mW/cm2的电磁波全身均匀辐射30min/d,连续7天,采用免疫组织化学方法检测小脑普肯耶细胞上GluR2及其磷酸化蛋白质的定位表达水平。结果电磁辐射后各时相点大鼠小脑GluR2及其磷酸化蛋白质的定位表达水平均未发生显著变化。结论短期接触低剂量的电磁辐射可能不会影响小脑内与学习记忆功能密切相关的GluR2的磷酸化过程。Objective To investigate alteration of cerebellar GluR2 and phosphorylation protein of rats after occupational electromag- netic exposure. Methods Sixty male wistar rats were randomly divided into exposure group and control group. Rats were exposed to 50mW/cm^2electromagnetic irradiation for thirty minutes everyday for seven days. The location of GluR2 and phosphorylated GluR2 were observed on purkinje cell membrane of cerebellum after immunohistochemistry. Results Both location and quantity of GluR2 and phos- phorylated GluR2 had no significantly alteration after electromagnetic exposure compared with control. Conclusion In this study, GluR2 phosphorylation of cerebellum has no significantly injury after little power and short term electromagnetic exposure.

关 键 词：电磁辐射 小脑 谷氨酸受体2 免疫组织化学 

分 类 号：R-332[医药卫生]