MDM2基因多态性与鲁西南汉族人群食管鳞癌风险关联  

Correlation of MDM2 gene polymorphisms with the risk of esophageal squamous cell carcinoma in Han nationalty of Southwest Shandong Province

作　　者：李睿[1] 张林[2] 刘湛[1] 候翠萍[3] 张业霞[1] 王宗军[1] 朱树国[3] 

机构地区：[1]菏泽医学专科学校,山东菏泽274030 [2]新乡医学院法医物证学教研室,河南新乡453003 [3]菏泽市立医院,山东菏泽274031

出　　处：《中国热带医学》2011年第9期1066-1068,共3页China Tropical Medicine

摘　　要：目的研究MDM2基因多态性在鲁西南汉族人群中的分布并分析其与食管鳞癌的相关性。方法采用聚合酶链反应一限制性内切酶片段长度多态性（FCR—RFLP）技术分析检测132食管鳞癌患者（病例组）和132非食管癌患者（对照组）MDM2SNP309的基因多态性，并比较不同基因型与食管鳞癌发病风险的关系。结果MDM2SNP309基因座位上突变等位基因频率在正常对照组和病例组分别为37．50％和45．45％，基因频率在两组间的分布无显著性差异（P〉0．05）；SNP309基因座中突变等位基因G的纯合子G／G基因型频率在对照组和病例组分别为10．61％和18．94％，频率差异具有统计学意义（P〈0．05）；MDM2SNP309的G／G基因型可增加食管鳞癌的发病风险（OR=2．27；95％CI：1．04～4．97）。结论MDM2基因SNP309位点的G／G基因型可能与鲁西南地区食管鳞癌的发病风险增高具有相关性。Objectlve To current study was designed to study the distribution of the polymorphisms of MDM-2 gene in Southwest Shandong Hans population and evaluate the association between MDM-2 gene polymorphisms and susceptibility of Esophageal Squamous Cell Carcinoma （ESCC）. Methods The polymorphisms of MDM2 at SNP309 position was analyzed by polymerase chain reaction-restriction fragment length polymorphism（PCR-RFLP） technique in 132 esophageal, squamous cell carcinoma cases and 132 non-cancer controls. The frequencies of genotype and allele and the associations between the genetic polymorphisms and cancer risk were analyzed. Results The polymorphism variants of MDM2 SNP309 in controls were 37.50% ,which in cases were 45.45% ,respectively. No significant difference was observed between cases and controls （P〉0.05）. The G/G genotype of SNP309 in control and case were 10.61% and 18.94% respectively,which have the significant difference between the two groups （P〈0.05） and make the ESCC risk increase statistically （OR=2.27 ;95%CI： 1.04-4.97）. Conclusion The G/G genotype of SNP309 may be an important hereditary factor which influence the occurrence of ESCC.

关 键 词：MDM2 SNP309 基因多态性 食管鳞癌 

分 类 号：R735.1[医药卫生—肿瘤]