生物柴油副产物粗甘油发酵生产γ-亚麻酸的条件优化  

Optimization of producing γ-linolenic acid from biodiesel by-product of crude glycerol

作　　者：姜翠红[1] 杨天奎[1] 牟英[1] 

机构地区：[1]大连理工大学生命科学与技术学院,辽宁大连116024

出　　处：《中国油脂》2011年第11期83-87,共5页China Oils and Fats

基　　金："863"计划项目(2010AA101505);国家自然科学基金(21106013)

摘　　要：以生物柴油副产物粗甘油为碳源,利用Box-Benhnken实验设计和响应面法探索了深黄被孢霉生产γ-亚麻酸(GLA)的条件。实验结果表明,以生物柴油副产物粗甘油为碳源培养深黄被孢霉生产GLA的最优条件为:尿素质量浓度1.0 g/L,培养温度27.5℃,培养时间12 d。在最优条件下深黄被孢霉干生物量为51.45 g/L,总脂量为40.99 g/L,GLA含量为1.94 g/L。Crude glycerol from biodiesel industry was used as carbon source, and the Box - Benhnken de- sign and response surface methodology were employed as experimental methods to optimize the conditions of producing y - linolenic acid by Mortierella isabellina fermentation. The results indicated that the opti- mal culture conditions were as follows ： urea concentration 1.0 g/L, fermentation temperature 27.5 ℃ and fermentation time 12 d, and under the optimal culture conditions, the dry biomass of Mortierella isa- bellina was 51.45 g/L, the total lipid content was 40.99 g/L, the content of GLA was 1.94 g/L.

关 键 词：Γ-亚麻酸 深黄被孢霉 生物柴油 粗甘油 响应面分析 

分 类 号：TQ645.6[化学工程—精细化工]