响应曲面法优化利迪链霉菌A02发酵培养基  

Optimization of Medium Components for Production of Natamycin by Streptomyces lydicus A02 with Response Surface Methodology

作　　者：花玉鹏[1,2] 文才艺[1] 刘伟成[2] 杨剑芳[2] 卢彩鸽[2] 

机构地区：[1]河南农业大学植物保护学院,郑州450002 [2]北京市农林科学院植物保护环境保护研究所,北京100097

出　　处：《中国生物防治学报》2011年第4期520-527,共8页Chinese Journal of Biological Control

基　　金：北京市科技计划(Z101105052010002);公益性行业(农业)科研专项(200903049-07);北京市农林科学院项目(2010A006)

摘　　要：为了提高纳他霉素新产生菌利迪链霉菌Streptomyces lydicus A02的发酵水平,采用部分因子法、最陡爬坡试验和中心组合试验相结合的方法对该菌株的发酵培养基进行了优化。试验结果表明,培养基中的玉米淀粉、棉籽精粉和葡萄糖是影响纳他霉素产量的主要因子,由所得响应曲面方程预测出这3个主要因子的质量浓度分别为52.7,31.7和11.7g?L-1时,纳他霉素产量达最大值1735.3mg?L-1;经摇瓶和30L发酵罐试验验证,该理论预测值与实测值无显著差异;优化后培养基的产能较基础发酵培养基提高了32.9%～34.8%。据此认为,响应面法对利迪链霉菌产纳他霉素发酵培养基的优化具有实效性,本研究为其高产发酵工艺的建立奠定了基础。In order to increase the yield ofnatamycin produced by Streptomyces lydicus A02, fermentation medium was optimized with methods of fractional factorial design, path of steepest ascent and central composite design. Results showed that corn starch, cotton seed flour and glucose were the major factors affecting natamycin production. According to the evaluation by the response surface model established in this study, the optimum mass concentrations of the three components in fermentation medium were 52.7, 31.7 and 11.7 g.L- 1, respectively, with the highest natamycin yield of 1735.3 mg.L-J. Fermentation experiments with shake flasks and 30 L fermentor verified that there was no statistical difference between the yield value forecasted by the model and that obtained in the tests, and the natamycin production was increased by 32.9%--34.8% in the optimized medium in comparison with basic fermentation medium. This result indicated that the optimal formula of the medium for natamycin production of S. lydicus established with response surface methodology was applicable and effective, and it will provide reliable data for design of industrial medium and establishment of fermentation process for natamycin produced by S. lydicus.

关 键 词：利迪链霉菌 纳他霉素 发酵培养基 响应面设计 

分 类 号：S482.292[农业科学—农药学] TQ920.6[农业科学—植物保护]