桶装水中铜绿假单胞菌检测方法的比较  

Comparison of Detection of Pseudomonas aeruginosa in Bottled Water

作　　者：张淑红[1] 吴清平[1] 徐晓可[1] 张菊梅[1] 潘宝怡[1] 彭飞艇[1] 

机构地区：[1]广东省菌种保藏与应用重点实验室,广东省微生物应用新技术公共实验室,广东省华南应用微生物重点实验室一省部共建国家重点实验室培育基地,广东省微生物研究所,广东广州510070

出　　处：《现代食品科技》2011年第11期1403-1405,1335,共4页Modern Food Science and Technology

基　　金：广东省科技计划项目(2009A080209002、2010B031000020)

摘　　要：分别采用传统国标GB/T 8538-2008方法、基于OprL基因的PCR方法和环介导等温扩增(LAMP)方法检测桶装水中的铜绿假单胞菌,比较三种方法的检测效果。结果显示,采集的46份市场水样中,用传统GB8538-2008方法检出了3份阳性样品,检出率为6.5%,检测时间至少2天;而PCR和LAMP方法都检出4份阳性样品,检出率为8.7%,并且可在24 h内报告结果。PCR和LAMP方法与传统方法相比具有快速灵敏等优点,可用于大批量水样的快速检测和结果初筛。Three methods including traditional method GB/T 8538-2008,PCR assay based on OprL gene and LAMP assay were used to detect the Pseudomonas aeruginosa in bottled water and the results were compared.It was found that 3 bottled water samples（6.5%） were positive detected by GB8538-2008 method,while 4 bottled water samples were detected positive by both PCR and LAMP methods with the positive rate of 8.7%.As for the test of time,the traditional method need at least 2 days to finish,compared to less than 24h using PCR and LAMP methods.Therefore,PCR and LAMP assays were more rapid and sensitive than traditional method,which may be used for rapid detection of large quantities of water samples.

关 键 词：铜绿假单胞菌 传统方法 PCR LAMP 

分 类 号：TS275.1[轻工技术与工程—农产品加工及贮藏工程]