CPP-ACP对发酵乳杆菌生长、黏附影响的实验研究  

EXPERIMENTAL STUDY OF THE CASEIN PHOSPHOPEPTIDE-AMORPHIC CALCIUM PHOSPHATE ON THE GROWTH AND ADHERENCE OF LACTOBACILLUS FERMENTUM

作　　者：刘兴容[1] 李艳萍[1] 徐皑[1] 

机构地区：[1]泸州医学院附属口腔医院,泸州646000

出　　处：《现代预防医学》2011年第22期4694-4695,4702,共3页Modern Preventive Medicine

基　　金：四川泸州市科委科技计划项目资助(01-14/343)

摘　　要：[目的]研究酪蛋白磷酸肽钙磷复合体(Casein phosphopeptide-Amorphic Calcium Phosphate,CPP-ACP)对发酵乳杆菌生长、黏附的影响,进一步探讨CPP-ACP的防龋机制。[方法]将发酵乳杆菌接种到BHI培养基,实验组中加入不同浓度(0.5%～5.0%(W/V))的CPP-ACP溶液,厌氧培养48h后,采用噻唑蓝(MTT)比色分析法测定细菌浓度的吸光度A值(λ=550nm)。采用唾液包被的羟磷灰石(Saliva-coated hydroxyapatite,S-HA)形成实验性膜,用不同浓度(0.5～5.0%(W/V))CPP-ACP处理S-HA,定量观察发酵乳杆菌在S-HA上的黏附情况。[结果]随CPP-ACP浓度的升高,发酵乳杆菌甲臜产物的二甲亚砜溶液的吸光度值降低,即发酵乳杆菌的活菌数减少(P﹤0.01)。发酵乳杆菌对经各实验浓度的CPP-ACP处理后的S-HA黏附能力明显下降,黏附量(cpm)随CPP-ACP浓度的升高而降低,黏附抑制率达100%。[结论]CPP-ACP对发酵乳杆菌的生长以及在S-HA上的黏附具有抑制作用,随CPP-ACP浓度的升高抑制作用增强。[Objective]To investigate the effects of casein phosphopeptide-amorphic calcium phosphate（CPP-ACP） on the growth and adherence of Lactobacillus fermentum and the possible anti-caries mechanism of CPP-ACP.[Methods]Lactobacillus fermentum was inoculated in BHI with different concentrations of CPP-ACP and grew in anaerobic condition at 37℃ for 48 hours.MTT method was used to measure the effects of CPP-ACP on the growth of Lactobacillus fermentum.The hydroxyapatites（HA）deads were coated by saliva to form saliva-coated hydroxyapatite（S-HA）,an in vitro adhesion model of experimental pellicle.Lactobacillus fermentum was radiolabeled with 3H-thymidine and different concentrations（0.5%-5.0%（W/V）of CPP-ACP pretreated S-HA.The numbers of 3H-thymidine labeled bacteria attached to S-HA were counted by scintillation counting.[Results]It was found that CPP-ACP could inhibit the growth and adherence of Lactobacillus fermentum.With the concentration of CPP-ACP increasing,the values of A（550nm）and cpm decreased（P﹤0.01）,and all of the inhibition rate of adherence arrived at 100%.[Conclusion]CPP-ACP had inhibitory effect on the growth and adherence of Lactobacillus fermentum,and the inhibitory effect enhanced with the increasing of CPP-ACP concentration.

关 键 词：CPP-ACP 发酵乳杆菌 生长 黏附 S-HA 

分 类 号：R78[医药卫生—口腔医学]