超高效液相色谱-串联质谱法测定鱼组织中的6种抗雌激素药物  

Determination of 6 antiestrogens in fish tissues by ultra performance liquid chromatography-tandem mass spectrometry

作　　者：肖甚圣[1] 杨奕[2] 张晶[2] 吴永宁[1,3] 邵兵[2] 

机构地区：[1]武汉工业学院食品科学与工程学院,湖北武汉430023 [2]北京市疾病预防控制中心食物中毒诊断溯源技术北京市重点实验室,北京100013 [3]中国疾病预防控制中心营养与食品安全所,北京100025

出　　处：《色谱》2011年第11期1055-1061,共7页Chinese Journal of Chromatography

基　　金：国家自然科学基金重点项目(No.20837003);北京市卫生系统高层次卫生技术人才培养计划项目

摘　　要：采用超高效液相色谱-串联质谱(UPLC-MS/MS)在多反应监测(MRM)模式下建立了鱼肉、鱼肝中6种抗雌激素类药物(托瑞米芬、氯米芬、他莫昔芬、雷洛昔芬、阿那曲唑、来曲唑)的检测方法。样品采用乙腈超声提取,上清液中加入适量水稀释提取液,经混合型阳离子交换(MCX)固相萃取柱富集、净化后进行UPLC-MS/MS分析。UPLC分离在ACQUITY UPLCTM BEH C18柱(100 mm×2.1 mm,1.7μm)上进行,以乙腈和0.1%甲酸水溶液为流动相进行梯度洗脱。6种抗雌激素药物的定量限(以信噪比为10计)为0.1～0.3μg/kg;4个加标水平的平均回收率为84.9%～112.2%,相对标准偏差为0.9%～14.3%。该方法可用于鱼肉、鱼肝中6种抗雌激素药物的痕量分析。A comprehensive analytical method based on ultra performance liquid chromatography-tandem mass spectrometry（UPLC-MS/MS） has been developed for the simultaneous determination of 6 antiestrogens（toremifene,clomiphene,tamoxifen,raloxifene,anastrozole and letrozole） in fish muscle and liver.The multi-reaction monitoring mode was employed for the determination.The homogeneous fish tissue samples were ultrasonically extracted with acetonitrile,and then the supernatants were diluted by water.The target compounds were concentrated and purified by a mixed-mode cationic-exchanger（MCX） cartridge,and then separated on an ACQUITY UPLC^TM BEH C18 column（100 mm×2.1 mm,1.7 μm） using a binary mobile phase gradient with water containing 0.1% formic acid and acetonitrile.The limits of quantification（LOQ,S/N=10） of the 6 antiestrogens were 0.1-0.3 μg/kg in muscle and liver samples.The average recoveries of target compounds（spiked at four concentration levels） based on internal standard calibration were in the range of 84.9%-112.2% with the relative standard deviations of 0.9%-14.3%.This method can be applied to the trace analysis of target drugs in fish muscle and liver samples.

关 键 词：固相萃取 超高效液相色谱-串联质谱 抗雌激素药物 鱼肉 鱼肝 

分 类 号：O658[理学—分析化学]