Prokaryotic Expression of Pseudomonas Aeruginosa Lipase Gene  

铜绿假单胞菌脂肪酶Lipase基因的原核表达(英文)

作　　者：张煜星[1,2] 武寒雪[2] 祝建波[2] 刘焕[3] 周鹏[1] 

机构地区：[1]中国热带农业科学院热带生物技术研究所国家重点实验室,海南海口571101 [2]石河子大学生命科学学院农业生物技术重点实验室,新疆石河子832003 [3]新疆农业科学院微生物应用研究所,新疆乌鲁木齐830091

出　　处：《Agricultural Science & Technology》2008年第5期59-62,共4页农业科学与技术（英文版）

基　　金：Supported by Subproject of"Development and Utilization of Plant Resources under Special Environment"from the National Project"863"(2007AA021401);Corps Doctoral Foundation of"Study on Transgenic Breeding Technology"(2006JC07)~~

摘　　要：[Objective] The aim of this study was to investigate the prokaryotic expression of pseudomonas aeruginosa Lipase gene.[Method]Lipase gene was amplified by PCR from the genome DNA of pseudomonas aeruginosa,and its nucleotide sequence was determined.The prokaryotic expression vector of Lipase gene was constructed by the gene recombination technique.The protein expression was induced for 4 hours by IPTG with the final concentration of 1.0 mmol/L,and then SDS-PAGE electrophoresis was analyzed.[Result]The sequence of mature peptides in Lipase gene cloned from pseudomonas aeruginosa had a 99.36% homology with that of pseudomonas aeruginosa lipase submitted in NCBI,so the prokaryotic expression vector of Lipase gene pET32a-Lip was successfully constructed.Furthermore,the results of SDS-PAGE electrophoresis showed that the target gene was expressed highly and effectively.[Conclusion]The cloned pseudomonas aeruginosa lipase with its signal peptide could be normally expressed in E.coli and also used for further study.[Objective] The aim of this study was to investigate the prokaryotic expression of pseudomonas aeruginosa Lipase gene.[Method]Lipase gene was amplified by PCR from the genome DNA of pseudomonas aeruginosa,and its nucleotide sequence was determined.The prokaryotic expression vector of Lipase gene was constructed by the gene recombination technique.The protein expression was induced for 4 hours by IPTG with the final concentration of 1.0 mmol/L,and then SDS-PAGE electrophoresis was analyzed.[Result]The sequence of mature peptides in Lipase gene cloned from pseudomonas aeruginosa had a 99.36% homology with that of pseudomonas aeruginosa lipase submitted in NCBI,so the prokaryotic expression vector of Lipase gene pET32a-Lip was successfully constructed.Furthermore,the results of SDS-PAGE electrophoresis showed that the target gene was expressed highly and effectively.[Conclusion]The cloned pseudomonas aeruginosa lipase with its signal peptide could be normally expressed in E.coli and also used for further study.

关 键 词：PSEUDOMONAS AERUGINOSA LIPASE PROKARYOTIC EXPRESSION 

分 类 号：S188[农业科学—农业基础科学]