Isolation and Expression Analysis of MaPRMT1 Gene in Banana  

香蕉MaPRMT1基因的分离及表达分析(英文)

作　　者：刘凡[1,2] 张建斌[1] 贾彩红[1] 杨景豪[1] 徐碧玉[1] 金志强[1] 

机构地区：[1]中国热带农业科学院热带生物技术研究所,海南海口571101 [2]海南大学农学院,海南儋州571737

出　　处：《Agricultural Science & Technology》2008年第3期70-74,102,共6页农业科学与技术（英文版）

基　　金：公益性行业(农业)科研专项(nyhyzx07-029)~~

摘　　要：[Objective] The aim of experiment was to lay molecular foundation for studying maturity mechanism of banana after harvest. [Method] The combined method of suppressing subtractive hybridization and cDNA micro-array were used to obtain cDNA segment of one PRMT gene in banana and the whole cDNA sequence of the gene was cloned.The bioinformatics analysis was operated on it,in addition, the expression profile analysis was conducted in different organs and different mature periods of banana.[Result] The whole length of cDNA in MaPRMT1 was 1 158 bp and possessed a complete open reading frame,which could encode 385 amino acids.It had high homology with PRMT in plant,containing one Methyltransf_1 domain.The MaPRMT1 gene was expressed in root,stem,leaf and fruit of banana and the expression levels in stem and leaf were relatively high.As the increase of days after harvest,the expression level declined gradually,however it reached maximum when ethylene release was biggest,then it declined.[Conclusion] MaPRMT1 belonged to the first kind of arginine methyltransferase and it was expressed differently in different organs and fruits at different mature periods.[Objective] The aim of experiment was to lay molecular foundation for studying maturity mechanism of banana after harvest. [Method] The combined method of suppressing subtractive hybridization and cDNA micro-array were used to obtain cDNA segment of one PRMT gene in banana and the whole cDNA sequence of the gene was cloned.The bioinformatics analysis was operated on it,in addition, the expression profile analysis was conducted in different organs and different mature periods of banana.[Result] The whole length of cDNA in MaPRMT1 was 1 158 bp and possessed a complete open reading frame,which could encode 385 amino acids.It had high homology with PRMT in plant,containing one Methyltransf_1 domain.The MaPRMT1 gene was expressed in root,stem,leaf and fruit of banana and the expression levels in stem and leaf were relatively high.As the increase of days after harvest,the expression level declined gradually,however it reached maximum when ethylene release was biggest,then it declined.[Conclusion] MaPRMT1 belonged to the first kind of arginine methyltransferase and it was expressed differently in different organs and fruits at different mature periods.

关 键 词：BANANA Protein ARGININE METHYLTRANSFERASE (PRMT) MUSA acu minata PRMT1(MaPRMT1) GENE differential expression Reverse transcriptase-polynerase chain reaction(RT-PCR) 

分 类 号：S668.1[农业科学—果树学]